Efficacy of the live attenuated Francisella tularensis vaccine (LVS) in a murine model of disease

被引：34

作者：

Green, M ^{[1
]}

Choules, G ^{[1
]}

Rogers, D ^{[1
]}

Titball, RW ^{[1
]}

机构：

[1] DSTL Porton Down, Salisbury SP4 0JQ, Wilts, England

来源：

VACCINE | 2005年 / 23卷 / 20期

关键词：

Francisella tularensis; live vaccine strain (LVS); efficacy;

D O I：

10.1016/j.vaccine.2004.03.071

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A live attenuated vaccine Francisella tularensis live vaccine strain (LVS), that confers protection against tularemia infection in a number of animal models including man was developed during the 1960s in the US. In this study, we have established the median lethal dose (MLD) after intraperitoneal (i.p.) or intravenous (i.v.) delivery of NDBR Lot 4 F. tularensis LVS to be 4 cfu and 2.24 x 10(4) cfu, respectively, in BALB/c mice and less than 1 cfu and 1.29 x 10(4) cfu, respectively, in C57BL/6 mice. When delivered subcutaneously, the MLD for F. tularensis LVS was greater then 1 x 10(8) cfu in both strains of mouse. Using mouse models of systemic tularemia infection it was demonstrated that F. tularensis LVS immunised BALB/c mice were fully protected after challenge with approximately 1000 MLD of a strain of F. tularensis subsp. tularensis or a strain of F. tularensis subsp. holarctica. Under similar challenge conditions, protection in C57BL/6 mice was only evident against a subsp. holarctica strain. In BALB/c mice, protection against a subsp. holarctica strain was achieved 4 days after F. tularensis LVS immunisation whereas protection against a subsp. tularensis strain was only evident 14 days after F. tularensis LVS immunisation. (c) 2004 Published by Elsevier Ltd.

引用

页码：2680 / 2686

页数：7

共 26 条

[1] EVALUATION OF LIVE TULAREMIA VACCINE PREPARED IN A CHEMICALLY DEFINED MEDIUM [J].