Fluctuation correlation spectroscopy with a laser-scanning microscope: Exploiting the hidden time structure

被引：156

作者：

Digman, MA

Sengupta, P

Wiseman, PW

Brown, CM

Horwitz, AR

Gratton, E ^{[1
]}

机构：

[1] Univ Illinois, Fluorescence Dynam Lab, Urbana, IL 61801 USA

[2] McGill Univ, Dept Chem & Phys, Montreal, PQ H3A 2K6, Canada

[3] Univ Virginia, Sch Med, Dept Cell Biol, Charlottesville, VA 22908 USA

来源：

BIOPHYSICAL JOURNAL | 2005年 / 88卷 / 05期

关键词：

D O I：

10.1529/biophysj.105.061788

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

引用

页码：L33 / L36

页数：4

共 7 条

[1] 2-PHOTON FLUORESCENCE CORRELATION SPECTROSCOPY - METHOD AND APPLICATION TO THE INTRACELLULAR ENVIRONMENT [J].