Peptide functionalized poly(L-lysine)-g-poly(ethylene glycol) on titanium:: resistance to protein adsorption in full heparinized human blood plasma

被引:177
作者
Tosatti, S
De Paul, SM
Askendal, A
VandeVondele, S
Hubbell, JA
Tengvall, P
Textor, M [1 ]
机构
[1] ETH, Swiss Fed Inst Technol, Dept Mat, Surface Sci & Technol Lab,BioInterfaceGrp, CH-8092 Zurich, Switzerland
[2] Univ Zurich, CH-8092 Zurich, Switzerland
[3] ETH, Inst Biomed Engn, Dept Mat, CH-8092 Zurich, Switzerland
[4] Linkoping Univ, Dept Phys & Measurement Technol, Div Appl Phys, SE-58183 Linkoping, Sweden
关键词
protein resistance; poly(ethylene glycol); graft copolymers; titanium; blood plasma; peptide;
D O I
10.1016/S0142-9612(03)00420-4
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The graft copolymer poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its RGD- and RDG-functionalized derivatives (PLL-g-PEG/PEG-peptide) were assembled from aqueous solutions on titanium (oxide) surfaces. The polymers were characterized by NMR in order to determine quantitatively the grafting ratio, g (Lys monomer umts/PEG side chains), and the fraction of the PEG side chains carrying the terminal peptide group. The titanium surfaces modified with the polymeric monomolecular adlayers were exposed to full heparinized blood plasma. The adsorbed masses were measured by in situ ellipsometry. The different PLL-g-PEG-coated surfaces showed, within the detection limit of the ellipsometric technique, no statistically significant protein adsorption during exposure to plasma for 30 min at 22degreesC or 37degreesC, whereas clean, uncoated titanium surfaces adsorbed approximately 350 ng/ cm 2 of plasma proteins. The high degree of resistance of the PEGylated surface to non-specific adsorption makes peptide-modified PLL-g-PEG a useful candidate for the surface modification of biomedical devices such as implants that are capable of eliciting specific interactions with integrin-type cell receptors even in the presence of full blood plasma. The results refer to short-term blood plasma exposure that cannot be extrapolated a priori to long-term clinical performance. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4949 / 4958
页数:10
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