Features of Tri101, the trichothecene 3-O-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum

被引:37
作者
Kimura, M
Shingu, Y
Yoneyama, K
Yamaguchi, I
机构
[1] Inst Phys & Chem Res, Microbial Toxicol Lab, Wako, Saitama 3510198, Japan
[2] Meiji Univ, Fac Agr, Plant Pathol Lab, Tama Ku, Kawasaki, Kanagawa 214, Japan
关键词
Fusarium mycotoxin; trichothecene biosynthesis; resistance gene; cosmid clones; transcription regulatory gene;
D O I
10.1271/bbb.62.1033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A structural gene of Tri101, which encodes trichothecene 3-O-acetyltransferase, was isolated as a 3-kb XTroI-XbaI fragment from the trichothecene producer Fusarium graminearum strain F15. The gene contained no introns, and the coding region was 0.7-kb downstream of a putative UTP-ammonia ligase gene which obviously is not related to the biosynthesis of trichothecenes. Tri101 was expressed when T-2 toxin was added, but this induction was not dependent on the expression level of Tri6, a transcription activator gene in the trichothecene biosynthetic and regulatory gene cluster.
引用
收藏
页码:1033 / 1036
页数:4
相关论文
共 15 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   TRANSCRIPTIONAL REGULATION OF BIALAPHOS BIOSYNTHESIS IN STREPTOMYCES-HYGROSCOPICUS [J].
ANZAI, H ;
MURAKAMI, T ;
IMAI, S ;
SATOH, A ;
NAGAOKA, K ;
THOMPSON, CJ .
JOURNAL OF BACTERIOLOGY, 1987, 169 (08) :3482-3488
[3]   PROTEIN-SYNTHESIS INHIBITION BY 8-OXO-12,13-EPOXYTRICHOTHECENES [J].
EHRLICH, KC ;
DAIGLE, KW .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 923 (02) :206-213
[4]   ISOLATION AND NUCLEOTIDE-SEQUENCE OF A SESQUITERPENE CYCLASE GENE FROM THE TRICHOTHECENE-PRODUCING FUNGUS FUSARIUM-SPOROTRICHIOIDES [J].
HOHN, TM ;
BEREMAND, PD .
GENE, 1989, 79 (01) :131-138
[5]   EVIDENCE FOR A GENE-CLUSTER INVOLVING TRICHOTHECENE-PATHWAY BIOSYNTHETIC GENES IN FUSARIUM-SPOROTRICHIOIDES [J].
HOHN, TM ;
MCCORMICK, SP ;
DESJARDINS, AE .
CURRENT GENETICS, 1993, 24 (04) :291-295
[6]   THE TRI4 GENE OF FUSARIUM SPOROTRICHIOIDES ENCODES A CYTOCHROME-P450 MONOOXYGENASE INVOLVED IN TRICHOTHECENE BIOSYNTHESIS [J].
HOHN, TM ;
DESJARDINS, AE ;
MCCORMICK, SP .
MOLECULAR AND GENERAL GENETICS, 1995, 248 (01) :95-102
[7]   Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins -: Cloning and characterization of Tri101 [J].
Kimura, M ;
Kaneko, I ;
Komiyama, M ;
Takatsuki, A ;
Koshino, H ;
Yoneyama, K ;
Yamaguchi, I .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (03) :1654-1661
[8]   THE BIALAPHOS RESISTANCE GENE (BAR) PLAYS A ROLE IN BOTH SELF-DEFENSE AND BIALAPHOS BIOSYNTHESIS IN STREPTOMYCES-HYGROSCOPICUS [J].
KUMADA, Y ;
ANZAI, H ;
TAKANO, E ;
MURAKAMI, T ;
HARA, O ;
ITOH, R ;
IMAI, S ;
SATOH, A ;
NAGAOKA, K .
JOURNAL OF ANTIBIOTICS, 1988, 41 (12) :1838-1845
[9]   Isolation and characterization of Tri3, a gene encoding 15-O-acetyltransferase from Fusarium sporotrichioides [J].
McCormick, SP ;
Hohn, TM ;
Desjardins, AE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :353-359
[10]   A CATALOG OF SPLICE JUNCTION SEQUENCES [J].
MOUNT, SM .
NUCLEIC ACIDS RESEARCH, 1982, 10 (02) :459-472