Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice

被引：47

作者：

Kato, Shigeki

Mohri, Yasuaki

Matsuo, Tsuyoshi

Ogawa, Eisaku

Umezawa, Akihiro

Okuyama, Ryuhei

Nishimori, Katsuhiko

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Mol Biol Lab, Aoba Ku, Sendai, Miyagi 9818555, Japan

[2] Tohoku Univ, Grad Sch Med, Dept Dermatol, Sendai, Miyagi 9808574, Japan

[3] Natl Res Inst Child Hlth & Dev, Setagaya Ku, Tokyo 1578535, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 24期

关键词：

LGR4; GPR48; GPCR; gene deletion mice; EOB; keratinocyte;

D O I：

10.1016/j.febslet.2007.08.064

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：4685 / 4690

页数：6

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