Reconstitution of chromatin complexes from high-performance liquid chromatography-purified histones

被引：33

作者：

Ausio, J ^{[1
]}

Moore, SC ^{[1
]}

机构：

[1] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC V8W 3P6, Canada

来源：

METHODS | 1998年 / 15卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/meth.1998.0637

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method to reconstitute chromatin complexes from reversed-phase high-performance liquid chromatography (HPLC)-purified histones. The complexes reconstituted in this way exhibit the same structural characteristics as their equivalent native counterparts. Furthermore, this method works independently of the acid- or salt-extracted origin of the histones used for the HPLC fractionation. The potential of this method for the reconstitution of chromatin particles consisting of sequence-defined DNA templates and well-defined histone variants and/or their posttranslationally modified isoforms is discussed. (C) 1998 Academic Press.

引用

页码：333 / 342

页数：10

共 57 条

[1]

[Anonymous], 1884, Z PHYSL CHEM

[2] USE OF SELECTIVELY TRYPSINIZED NUCLEOSOME CORE PARTICLES TO ANALYZE THE ROLE OF THE HISTONE TAILS IN THE STABILIZATION OF THE NUCLEOSOME [J].

AUSIO, J ;

DONG, F ;

VANHOLDE, KE .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 206 (03) :451-463

[3]

AUSIO J, 1992, MOL CELL BIOCHEM, V115, P163

[4] NUCLEOSOME CORE PARTICLE STABILITY AND CONFORMATIONAL CHANGE - EFFECT OF TEMPERATURE, PARTICLE AND NACL CONCENTRATIONS, AND CROSSLINKING OF HISTONE-H3 SULFHYDRYL-GROUPS [J].

AUSIO, J ;

SEGER, D ;

EISENBERG, H .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 176 (01) :77-104

[5] NUCLEOSOME ASSEMBLY OF SIMIAN VIRUS-40 DNA IN A MAMMALIAN-CELL EXTRACT [J].

BANERJEE, S ;

CANTOR, CR .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (06) :2863-2873

[6] CELL-FREE SYSTEM FOR ASSEMBLY OF TRANSCRIPTIONALLY REPRESSED CHROMATIN FROM DROSOPHILA EMBRYOS [J].

BECKER, PB ;

WU, C .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (05) :2241-2249

[7] INTERACTION OF CORE HISTONES WITH DNA - EQUILIBRIUM BINDING STUDIES [J].

BURTON, DR ;

BUTLER, MJ ;

HYDE, JE ;

PHILLIPS, D ;

SKIDMORE, CJ ;

WALKER, IO .

NUCLEIC ACIDS RESEARCH, 1978, 5 (10) :3643-3663

[8] REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF HISTONES [J].

CERTA, U ;

VONEHRENSTEIN, G .

ANALYTICAL BIOCHEMISTRY, 1981, 118 (01) :147-154

[9] NUCLEOSOME DISSOCIATION AT PHYSIOLOGICAL IONIC STRENGTHS [J].

COTTON, RW ;

HAMKALO, BA .

NUCLEIC ACIDS RESEARCH, 1981, 9 (02) :445-457

[10] Determination of molecular parameters by fitting sedimentation data to finite-element solutions of the Lamm equation [J].

Demeler, B ;

Saber, H .

BIOPHYSICAL JOURNAL, 1998, 74 (01) :444-454

← 1 2 3 4 5 6 →