Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity

被引：514

作者：

Gruss, OJ ^{[1
]}

Carazo-Salas, RE ^{[1
]}

Schatz, CA ^{[1
]}

Guarguaglini, G ^{[1
]}

Kast, J ^{[1
]}

Wilm, M ^{[1
]}

Le Bot, N ^{[1
]}

Vernos, I ^{[1
]}

Karsenti, E ^{[1
]}

Mattaj, IW ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

CELL | 2001年 / 104卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(01)00193-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The small GTPase Ran, bound to GTP, is required for the induction of spindle formation by chromosomes in M phase. High concentrations of Ran.GTP are proposed to surround M phase chromatin. We show that the action of Ran.GTP in spindle formation requires TPX2, a microtubule-associated protein previously known to target a motor protein, Xklp2, to microtubules. TPX2 is normally inactivated by binding to the nuclear import factor, importin alpha, and is displaced from importin alpha by the action of Ran.GTP. TPX2 is required for Ran.GTP and chromatin-induced microtubule assembly in M phase extracts and mediates spontaneous microtubule assembly when present in excess over free importin alpha. Thus, components of the nuclear transport machinery serve to regulate spindle formation in M phase.

引用

页码：83 / 93

页数：11

共 55 条

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