Overlaps in the transcriptional profiles of Medicago truncatula roots inoculated with two different Glomus fungi provide insights into the genetic program activated during arbuscular mycorrhiza

被引:245
作者
Hohnjec, N
Vieweg, ME
Pühler, A
Becker, A
Küster, H
机构
[1] Univ Bielefeld, Fak Biol, Lehrstuhl Genet, D-33615 Bielefeld, Germany
[2] Univ Bielefeld, Ctr Biotechnol, Inst Genome Res, D-33594 Bielefeld, Germany
[3] Univ Bielefeld, Ctr Biotechnol, Int Grad Sch Bioinformat & Genome Res, D-33594 Bielefeld, Germany
关键词
D O I
10.1104/pp.104.056572
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting and various stress conditions. In this study, we focused on the overlapping genetic program activated by two commonly studied microsymbionts in addition to identifying AM-related genes. We thus applied 16,086 probe microarrays to profile the transcriptome of the model legume Medicago truncatula during interactions with Glomus mosseae and Glomus intraradices and specified a total of 201 plant genes as significantly coinduced at least 2-fold, with more than 160 being reported as AM induced for the first time. Several hundred genes were additionally up-regulated during a sole interaction, indicating that the plant genetic program activated in AM to some extent depends on the colonizing micro-symbiont. Genes induced during both interactions specified AM-related nitrate, ion, and sugar transporters, enzymes involved in secondary metabolism, proteases, and Kunitz-type protease inhibitors. Furthermore, coinduced genes encoded receptor kinases and other components of signal transduction pathways as well as AM-induced transcriptional regulators, thus reflecting changes in signaling. By the use of reporter gene expression, we demonstrated that one member of the AM-induced gene fan-Lily encoding blue copper binding proteins (MtBcpl) was both specifically and strongly up-regulated in arbuscule-containing regions of mycorrhizal roots. A comparison of the AM expression profiles to those of nitrogen-fixing root nodules suggested only a limited overlap between the genetic programs orchestrating root endosymbioses.
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页码:1283 / 1301
页数:19
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