The Drosophila homolog of Aut1 is essential for autophagy and development

被引：94

作者：

Juhász, G

Csikós, G

Sinka, R

Erdélyi, M

Sass, M

机构：

[1] Eotvos Lorand Univ, Dept Gen Zool, H-1117 Budapest, Hungary

[2] Biol Res Ctr, H-6701 Szeged, Hungary

来源：

FEBS LETTERS | 2003年 / 543卷 / 1-3期

关键词：

Drosophila; autophagy; fat body; Aut1/Apg3; CG6877/Draut1; RNA interference;

D O I：

10.1016/S0014-5793(03)00431-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：154 / 158

页数：5

共 24 条

[1] ULTRASTRUCTURE OF THE PREPARATIVE PHASE OF CELL-DEATH IN THE LARVAL FAT-BODY OF DROSOPHILA-MELANOGASTER [J].