Genetic evidence for an activator required for induction of colicin-like bacteriocin 28b production in Serratia marcescens by DNA-damaging agents

被引:28
作者
Ferrer, S [1 ]
Viejo, MB [1 ]
Guasch, JF [1 ]
Enfedaque, J [1 ]
Regue, M [1 ]
机构
[1] UNIV BARCELONA, FAC PHARM, DIV HLTH SCI, DEPT MICROBIOL & PARASITOL, E-08028 BARCELONA, SPAIN
关键词
D O I
10.1128/jb.178.4.951-960.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bacteriocin 28b production is induced by mitomycin in wild-type Serratia marcescens 2170 but not in Escherichia coli harboring the bacteriocin 28b structural gene (bss). Studies with a bss-lacZ transcriptional fusion showed that mitomycin increased the level of bss gene transcription in S. marcescens but not in the E. coli background, A S. marcescens Tn5 insertion mutant was obtained (S. marcescens 2170 reg::Tn5) whose bacteriocin 28b production and bss gene transcription were not increased by mitomycin treatment, Cloning and DNA sequencing of the mutated region showed that the Tn5 insertion was flanked by an SOS box sequence and three genes that are probably cotranscribed (regA, regB, and regC), These three genes had homology to phage holins, phage lysozymes, and the Ogr transcriptional activator of P2 and related bacteriophages, respectively, Recombinant plasmid containing this wild-type DNA region complemented the reg::TnS regulatory mutant, A transcriptional fusion between a 157-bp DNA fragment, containing the apparent SOS box upstream of the regA gene, and the caf gene showed increased chloramphenicol acetyltransferase activity upon mitomycin treatment, Upstream of the bss gene, a sequence similar to the consensus sequence proposed to bind Ogr protein was found, but no sequence similar to an SOS box was detected. Our results suggest that transcriptional induction of bacteriocin 28b upon mitomycin treatment is mediated by the regC gene whose own transcription would be LexA dependent.
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页码:951 / 960
页数:10
相关论文
共 58 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]  
[Anonymous], [No title captured]
[3]   STRUCTURAL-ANALYSIS OF TN5 [J].
AUERSWALD, EA ;
LUDWIG, G ;
SCHALLER, H .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1980, 45 :107-113
[4]   EXPRESSION OF SERRATIA-MARCESCENS EXTRACELLULAR PROTEINS REQUIRES RECA [J].
BALL, TK ;
WASMUTH, CR ;
BRAUNAGEL, SC ;
BENEDIK, MJ .
JOURNAL OF BACTERIOLOGY, 1990, 172 (01) :342-349
[5]   ATTACHMENT SITES FOR BACTERIOPHAGE-P2 ON THE ESCHERICHIA-COLI CHROMOSOME - DNA-SEQUENCES, LOCALIZATION ON THE PHYSICAL MAP, AND DETECTION OF A P2-LIKE REMNANT IN ESCHERICHIA-COLI K-12 DERIVATIVES [J].
BARREIRO, V ;
HAGGARDLJUNGQUIST, E .
JOURNAL OF BACTERIOLOGY, 1992, 174 (12) :4086-4093
[6]   COLIPHAGE-P2 LATE CONTROL GENE OGR DNA-SEQUENCE AND PRODUCT IDENTIFICATION [J].
BIRKELAND, NK ;
LINDQVIST, BH .
JOURNAL OF MOLECULAR BIOLOGY, 1986, 188 (03) :487-490
[7]  
BLASBAND AJ, 1986, J BIOL CHEM, V261, P2723
[8]   DUAL START MOTIF IN 2 LAMBDOID S-GENES UNRELATED TO LAMBDA-S [J].
BONOVICH, MT ;
YOUNG, R .
JOURNAL OF BACTERIOLOGY, 1991, 173 (09) :2897-2905
[9]   CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].
CHANG, ACY ;
COHEN, SN .
JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156
[10]   INTERACTIONS BETWEEN SATELLITE BACTERIOPHAGE-P4 AND ITS HELPERS [J].
CHRISTIE, GE ;
CALENDAR, R .
ANNUAL REVIEW OF GENETICS, 1990, 24 :465-490