High-performance capillary electrophoretic characterization of different types of oligo- and polysialic acid chains

被引:30
作者
Cheng, MC [1 ]
Lin, SL [1 ]
Wu, SH [1 ]
Inoue, S [1 ]
Inoue, Y [1 ]
机构
[1] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan
关键词
oligo/polysialic acids; alpha 2 -> 8-linked oligo/polyNeu5Ac; oligo/polyNeu5Ge and oligo/polyKDN; alpha 2 -> 5-O-glycolyl-linked oligo/polyNeu5Gc; capillary electrophoresis;
D O I
10.1006/abio.1998.2701
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have carried out comparative structural analysis of novel oligo- and polysialic acid chains from diverse sources. Controlled acid hydrolysates of (a) colominic acid, alpha 2-->8-linked homopolymer of N-acetylneuraminic acid (Neu5Ac), (b) alpha 2-->8-linked oligo/polyNeu5Gc chains present in rainbow trout egg polysialoglycoprotein, and (c) alpha 2-->8-linked oligomers of deaminoneuraminic acid (KDN) residues of KDN-rich glycoprotein derived from rainbow trout vitelline envelope were analyzed by highperformance capillary electrophoresis (HPCE). The results showed that three different types of alpha 2-->8-linked oligosialic acids having same degree of polymerization can be separated by HPCE. A partial hydrolysate of colominic acid with mild acid was shown by CE to form intramolecular esters during the controlled hydrolysis and the subsequent workup procedure. In contrast, lactonization of (-->5-O-glycolyl-Neu5Gc alpha 2-->)n, alpha 2-->5-O-glycolyl-linked homopolymer of N-glycolylneuraminic acid (Neu5Gc) present in the egg jelly coat of sea urchin, did not take place as readily as in (-->8Neu5Ac alpha 2-->)n. (C) 1998 Academic Press.
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页码:154 / 159
页数:6
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