ctr1, a gene involved in a signal transduction pathway of the gliding motility in the cyanobacterium Synechocystis sp PCC 6803

被引:26
作者
Chung, YH
Cho, MS
Moon, YJ
Choi, JS
Yoo, YC
Park, YI
Lee, KM
Kang, KW
Park, YM
机构
[1] Korea Basic Sci Inst, Biomol Res Team, Taejon 305333, South Korea
[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
[3] Chungnam Natl Univ, Dept Biol, Taejon 305764, South Korea
基金
新加坡国家研究基金会;
关键词
methyl-accepting chemotaxis protein; gliding motility; pilus production; transposon mutagenesis; Synechocystis; 6803;
D O I
10.1016/S0014-5793(01)02227-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We generated random Tn5 mutations in Synechocystis sp, PCC 6803 in search for genus involved in the signal transduction cascade for the cyanobacterial gliding motility. One of the non-gliding Tn5 mutants, S1-105, had an insertional inactivation in the slr1044 gene encoding a putative methyl-accepting chemotaxis protein. Interposon mutation on the slr1044 (named ctr1) in the bacterium also eliminated gliding motility. In the interposon mutant, the expression of pilA1 was 5-fold decreased compared with that of wild-type and thick pill, that are believed to he the motor for gliding, could not he observed by an electron microscope. Therefore, we suggest that the Ctr1 protein functions as a transducer that regulates the expression of pilA1, and thus is required for the biogenesis of thick pili. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B,V. All rights reserved.
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页码:33 / +
页数:134
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