Evidence that lipopolysaccharide-induced cell death is mediated by accumulation of reactive oxygen species and activation of p38 in rat cortex and hippocampus

Lipopolysaccharide (LPS) administration stimulates immune activation, inflammation and deterioration in cell function. Neuronal tissue in cortex and hippocampus are particularly susceptible. In this study, we report that LPS induces cell death as measured by caspase-3 activation and DNA fragmentation and that this is coupled with stimulation of the mitogen-activated protein kinase, p38. We provide evidence of colocalization of activated p38 and caspase-3 in cells prepared from cortical and hippocampal tissue after LPS treatment. Furthermore, administration of the p38 inhibitor, SB203580, abolished the LPS-induced increase in caspase-3 activation. We observed that LPS treatment provoked accumulation of reactive oxygen species (ROS) while in vitro incubation of cortical and hippocampal tissue with H202 increased p38 activity. In addition, H2O2-induced activation of caspase-3 was abrogated by SB203580. We propose, based on the data presented, that the action of LPS to induce cell death in cortex and hippocampus may be mediated by ROS accumulation and activation of p38. (C) 2003 Elsevier Inc. All rights reserved.