High throughput human DNA purification with aminosilanes tailored silica-coated magnetic nanoparticles

被引:42
作者
Park, Moo Eon [1 ]
Chang, Jeong Ho [1 ]
机构
[1] Korea Inst Ceram Engn & Technol, Seoul 153801, South Korea
来源
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2007年 / 27卷 / 5-8期
关键词
magnetic nanoparticle; silica shell; human DNA; purification;
D O I
10.1016/j.msec.2006.09.008
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
This work described the development of high throughput human DNA purification process with the amino-functionalized silica coated magnetic nanoparticles. The magnetic nanoparticles were synthesized with average particle size of 9 nm and silica-coated magnetic nanoparticles were obtained by controlling the coating thicknesses on magnetic nanoparticles. The silica coating thickness could be uniform-sized in the diameter of 10-40 nm by a sot-gel approach. The surface modification was performed with amino-functionalized organic silanes on silica coated magnetic nanoparticles. The spectroscopic measurements. such as a FT-IR (ATR-method) and Vibrational Sample Magnetometer (VSM) were used to characterize the chemical structures and magnetic strengths. To elucidate the relationship among the surface area, pore size distribution and reactivity of the materials, XRD, TEM, BET and Zeta potential were used. The use of functionalized self-assembled magnetic nanoparticles for human DNA separation process gives a lot of advantages rather than the conventional silica based process. (C) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1232 / 1235
页数:4
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