The effect of body temperature on the murine electroretinogram

被引：28

作者：

Kong, H ^{[1
]}

Gouras, P ^{[1
]}

机构：

[1] Columbia Univ, Dept Ophthalmol, New York, NY 10032 USA

来源：

DOCUMENTA OPHTHALMOLOGICA | 2003年 / 106卷 / 03期

关键词：

body temperature; murine ERG;

D O I：

10.1023/A:1022988332578

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: To study the effect of body temperature on the murine electroretinogram (ERG). Methods: The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37degreesC. Results: ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37degreesC. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 muV/degree. At 30degreesC, maximum b-wave amplitude was about 400 muV; at 37degreesC it was about 1000 muV. A change in body temperature produced a rapid change in ERG amplitude. Conclusion: The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.

引用

页码：239 / 242

页数：4

共 14 条

[1] TEMPORAL TRANSFER AND NONLINEARITY PROPERTIES OF TURTLE ERG - TUNING BY TEMPERATURE, PHARMACOLOGY, AND LIGHT-INTENSITY [J].