Activity and Diversity of Methanotrophic Bacteria at Methane Seeps in Eastern Lake Constance Sediments

被引：45

作者：

Deutzmann, Joerg S. ^{[1
]}

Woerner, Susanne ^{[1
]}

Schink, Bernhard ^{[1
]}

机构：

[1] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 08期

关键词：

RICE FIELD; MOLECULAR CHARACTERIZATION; PROFUNDAL SEDIMENTS; SURFACE-LAYER; COMMUNITY; POCKMARKS; OXIDATION; SOIL; SUCCESSION; ABUNDANCE;

D O I：

10.1128/AEM.02776-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The activity and community structure of aerobic methanotrophic communities were investigated at methane seeps (pockmarks) in the littoral and profundal zones of an oligotrophic freshwater lake (Lake Constance, Germany). Measurements of potential methane oxidation rates showed that sediments inside littoral pockmarks are hot spots of methane oxidation. Potential methane oxidation rates at littoral pockmark sites exceeded the rates of the surrounding sediment by 2 orders of magnitude. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the pmoA gene revealed major differences in the methanotrophic community composition between littoral pockmarks and the surrounding sediments. Clone library analysis confirmed that one distinct Methylobacter-related group dominates the community at littoral pockmarks. In profundal sediments, the differences between pockmarks and surrounding sediments were found to be less pronounced.

引用

页码：2573 / 2581

页数：9

共 50 条

[1]

GROWTH OF METHANOTROPHS IN METHANE AND OXYGEN COUNTER GRADIENTS [J].

AMARAL, JA ;

KNOWLES, R .

FEMS MICROBIOLOGY LETTERS, 1995, 126 (03) :215-220

[2]

[Anonymous], 2010, METHANE CLIMATE CHAN

[3]

Molecular characterization of methanotrophic isolates from freshwater lake sediment [J].

Auman, AJ ;

Stolyar, S ;

Costello, AM ;

Lidstrom, ME .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (12) :5259-5266

[4]

Methane emissions from lakes: Dependence of lake characteristics, two regional assessments, and a global estimate [J].

Bastviken, D ;

Cole, J ;

Pace, M ;

Tranvik, L .

GLOBAL BIOGEOCHEMICAL CYCLES, 2004, 18 (04) :1-12

[5]

BOSSE U, 1993, FEMS MICROBIOL ECOL, V13, P123, DOI 10.1111/j.1574-6941.1993.tb00058.x

[6]

Comparison of pmoA PCR primer sets as tools for investigating methanotroph diversity in three Danish soils [J].

Bourne, DG ;

McDonald, IR ;

Murrell, JC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (09) :3802-3809

[7]

Preferential cultivation of type II methanotrophic bacteria from littoral sediments (Lake Constance) [J].

Bussmann, I ;

Pester, M ;

Brune, A ;

Schink, B .

FEMS MICROBIOLOGY ECOLOGY, 2004, 47 (02) :179-189

[8]

Active pockmarks in a large lake (Lake Constance, Germany): Effects on methane distribution and turnover in the sediment [J].

Bussmann, Ingeborg ;

Schloemer, Stefan ;

Schlueter, Michael ;

Wessels, Martin .

LIMNOLOGY AND OCEANOGRAPHY, 2011, 56 (01) :379-393

[9]

The global methane cycle: recent advances in understanding the microbial processes involved [J].

Conrad, Ralf .

ENVIRONMENTAL MICROBIOLOGY REPORTS, 2009, 1 (05) :285-292

[10]

Costello AM, 1999, APPL ENVIRON MICROB, V65, P5066

← 1 2 3 4 5 →