Biochemical and molecular characterization of a putative endoglucanase in Magnaporthe grisea

被引:13
作者
Zhou, Jie [1 ,2 ]
Zheng, Xiang-zi [2 ]
Lan, Lan [2 ]
Lin, Chen-zeng [2 ]
Wu, Yu-bing [2 ]
Lin, Xiong-jie [1 ]
Ebbole, Daniel [3 ]
Lu, Guo-dong [1 ]
Wang, Zong-hua [1 ,2 ]
机构
[1] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, Fuzhou 350002, Peoples R China
[2] Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China
[3] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA
基金
美国国家科学基金会;
关键词
Magnaporthe grisea; endo-1; 4-beta-glucanase; secretory protein; pathogenicity;
D O I
10.1007/s00294-008-0179-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysis indicates that the protein encoded by gene MGG_02532.5 in M. grisea (named MgEGL1 for (M) under bar. (g) under bar risea (e) under bar ndo (g) under bar lucanase (1) under bar) contains a secretory signal peptide. Multiple alignment shows that MgEGL1 has high level of homology to endoglucanases (EC 3.1.1.4) from Aspergillus nidulans and Trichoderma reesei. The three proteins share a conserved catalytic domain, but only the one from T. reesei contains a cellulose binding module. MgEGL1 was constitutively expressed with the highest level in mycelia and the lowest in conidia. Interestingly, the MgEGL1 RNA could be alternatively processed when cultured in vitro and after infection of rice. Expression analysis confirmed that the MgEGL1 is a secreted protein. Its endoglucanase activity was assayed by Congo red plates, and further confirmed by the dinitrosalicylic acid method. The finding in this paper will provide the basis for further determination of the biochemical properties of the endoglucanase protein and its relevant function in fungal pathogenesis.
引用
收藏
页码:217 / 224
页数:8
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