Genetic manipulation of spirochetes - light at the end of the tunnel

被引：17

作者：

Cabello, FC ^{[1
]}

Sartakova, ML ^{[1
]}

Dobrikova, EY ^{[1
]}

机构：

[1] New York Med Coll, Dept Microbiol & Immunol, Valhalla, NY 10595 USA

来源：

TRENDS IN MICROBIOLOGY | 2001年 / 9卷 / 06期

关键词：

D O I：

10.1016/S0966-842X(01)02046-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spirochetes cause many important diseases in humans and animals, including syphilis, relapsing fever, leptospirosis and Lyme disease. Recent advances in molecular genetics have allowed Koch's molecular postulates to be applied to the study of the virulence factors in these bacteria. Such advances include the use of electroporation, the identification of several antibiotic resistance phenotypes as useful genetic markers,the development of extrachromosomal cloning vectors and the isolation of knockout m uta nts a nd their complementation.

引用

页码：245 / 248

页数：4

共 24 条

[1] Efficient targeted mutagenesis in Borrelia burgdorferi [J].

Bono, JL ;

Elias, AF ;

Kupko, J ;

Stevenson, B ;

Tilly, K ;

Rosa, P .

JOURNAL OF BACTERIOLOGY, 2000, 182 (09) :2445-2452

[2] In vivo genetic analysis of bacterial virulence [J].

Chiang, SL ;

Mekalanos, JJ ;

Holden, DW .

ANNUAL REVIEW OF MICROBIOLOGY, 1999, 53 :129-154

[3] Altered stationary-phase response in a Borrelia burgdorferi ropS mutant [J].

Elias, AF ;

Bono, JL ;

Carroll, JA ;

Stewart, P ;

Tilly, K ;

Rosa, P .

JOURNAL OF BACTERIOLOGY, 2000, 182 (10) :2909-2918

[4]

FALKOW S, 1988, REV INFECT DIS, V10, pS274

[5] Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi [J].

Fraser, CM ;

Casjens, S ;

Huang, WM ;

Sutton, GG ;

Clayton, R ;

Lathigra, R ;

White, O ;

Ketchum, KA ;

Dodson, R ;

Hickey, EK ;

Gwinn, M ;

Dougherty, B ;

Tomb, JF ;

Fleischmann, RD ;

Richardson, D ;

Peterson, J ;

Kerlavage, AR ;

Quackenbush, J ;

Salzberg, S ;

Hanson, M ;

vanVugt, R ;

Palmer, N ;

Adams, MD ;

Gocayne, J ;

Weidman, J ;

Utterback, T ;

Watthey, L ;

McDonald, L ;

Artiach, P ;

Bowman, C ;

Garland, S ;

Fujii, C ;

Cotton, MD ;

Horst, K ;

Roberts, K ;

Hatch, B ;

Smith, HO ;

Venter, JC .

NATURE, 1997, 390 (6660) :580-586

[6] Complete genome sequence of Treponema pallidum, the syphilis spirochete [J].

Fraser, CM ;

Norris, SJ ;

Weinstock, CM ;

White, O ;

Sutton, GG ;

Dodson, R ;

Gwinn, M ;

Hickey, EK ;

Clayton, R ;

Ketchum, KA ;

Sodergren, E ;

Hardham, JM ;

McLeod, MP ;

Salzberg, S ;

Peterson, J ;

Khalak, H ;

Richardson, D ;

Howell, JK ;

Chidambaram, M ;

Utterback, T ;

McDonald, L ;

Artiach, P ;

Bowman, C ;

Cotton, MD ;

Fujii, C ;

Garland, S ;

Hatch, B ;

Horst, K ;

Roberts, K ;

Sandusky, M ;

Weidman, J ;

Smith, HO ;

Venter, JC .

SCIENCE, 1998, 281 (5375) :375-388

[7] Purification and characterization of VSH-1, a generalized transducing bacteriophage of Serpulina hyodysenteriae [J].

Humphrey, SB ;

Stanton, TB ;

Jensen, NS ;

Zuerner, RL .

JOURNAL OF BACTERIOLOGY, 1997, 179 (02) :323-329

[8] Cytoplasmic filament-deficient mutant of Treponema denticola has pleiotropic defects [J].

Izard, J ;

Samsonoff, WA ;

Limberger, RJ .

JOURNAL OF BACTERIOLOGY, 2001, 183 (03) :1078-1084

[9] Development of a gene transfer system in Treponema denticola by electroporation [J].

Li, H ;

Kuramitsu, HK .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 1996, 11 (03) :161-165

[10] Gene inactivation in the oral spirochete Treponema denticola: Construction of an flgE mutant [J].

Li, H ;

Ruby, J ;

Charon, N ;

Kuramitsu, H .

JOURNAL OF BACTERIOLOGY, 1996, 178 (12) :3664-3667

← 1 2 3 →