High resolution time-domain fluorescence lifetime imaging for biomedical applications

被引:44
作者
Dowling, K
Dayel, MJ
Hyde, SCW
French, PMW
Lever, MJ
Hares, JD
Dymoke-Bradshaw, AKL
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Femtosecond Opt Grp, London SW7 2BZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol & Med Syst, London SW7 2BY, England
[3] Kentech Instruments Ltd, Unit 9, Didcot OX11 9AG, Oxon, England
关键词
D O I
10.1080/095003499149872
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We report the development of a whole-field fluorescence lifetime imaging (FLIM) system with high temporal resolution based on a time-gated image intensifier. The optimized temporal gate width is <100 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences less than 10ps, have been imaged. The versatility of this FLIM system has been demonstrated by measuring both the temporal and spectral profiles of multiple fluorescent samples in a single acquisition. Initial fluorescence lifetime images suggest that this technique can provide a means of distinguishing between different tissue constituents.
引用
收藏
页码:199 / 209
页数:11
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