High resolution time-domain fluorescence lifetime imaging for biomedical applications

被引：44

作者：

Dowling, K

Dayel, MJ

Hyde, SCW

French, PMW

Lever, MJ

Hares, JD

Dymoke-Bradshaw, AKL

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Femtosecond Opt Grp, London SW7 2BZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol & Med Syst, London SW7 2BY, England

[3] Kentech Instruments Ltd, Unit 9, Didcot OX11 9AG, Oxon, England

来源：

JOURNAL OF MODERN OPTICS | 1999年 / 46卷 / 02期

关键词：

D O I：

10.1080/095003499149872

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report the development of a whole-field fluorescence lifetime imaging (FLIM) system with high temporal resolution based on a time-gated image intensifier. The optimized temporal gate width is <100 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences less than 10ps, have been imaged. The versatility of this FLIM system has been demonstrated by measuring both the temporal and spectral profiles of multiple fluorescent samples in a single acquisition. Initial fluorescence lifetime images suggest that this technique can provide a means of distinguishing between different tissue constituents.

引用

收藏

页码：199 / 209

页数：11

相关论文

共 15 条

[1] TIME-GATED IMAGING-SYSTEM FOR TUMOR-DIAGNOSIS [J].

CUBEDDU, R ;

TARONI, P ;

VALENTINI, G .

OPTICAL ENGINEERING, 1993, 32 (02) :320-325

[2] Time-resolved fluorescence and photon migration studies in biomedical and model random media [J].

Das, BB ;

Liu, F ;

Alfano, RR .

REPORTS ON PROGRESS IN PHYSICS, 1997, 60 (02) :227-292

[3] 2-D fluorescence lifetime imaging using a time-gated image intensifier [J].

Dowling, K ;

Hyde, SCW ;

Dainty, JC ;

French, PMW ;

Hares, JD .

OPTICS COMMUNICATIONS, 1997, 135 (1-3) :27-31

[4] ARGON-ION-PUMPED AND DIODE-PUMPED ALL-SOLID-STATE FEMTOSECOND CRLISRALF6 REGENERATIVE AMPLIFIERS [J].

HYDE, SCW ;

BARRY, NP ;

MELLISH, R ;

FRENCH, PMW ;

TAYLOR, JR ;

VANDERPOEL, CJ ;

VALSTER, A .

OPTICS LETTERS, 1995, 20 (02) :160-162

[5] FLUORESCENCE LIFETIME IMAGING OF INTRACELLULAR CALCIUM IN COS CELLS USING QUIN-2 [J].

LAKOWICZ, JR ;

SZMACINSKI, H ;

LEDERER, WJ ;

KIRBY, MS ;

JOHNSON, ML ;

NOWACZYK, K .

CELL CALCIUM, 1994, 15 (01) :7-27

[6] DIODE-PUMPED CR-LISAF ALL-SOLID-STATE FEMTOSECOND OSCILLATOR AND REGENERATIVE AMPLIFIER [J].

MELLISH, R ;

BARRY, NP ;

HYDE, SCW ;

JONES, R ;

FRENCH, PMW ;

TAYLOR, JR ;

VANDERPOEL, CJ ;

VALSTER, A .

OPTICS LETTERS, 1995, 20 (22) :2312-2314

[7] Direct and fast uranium determination in zirconium by time-resolved laser-induced fluorescence spectrometry [J].

Moulin, C ;

Decambox, P ;

Trecani, L .

ANALYTICA CHIMICA ACTA, 1996, 321 (01) :121-126

[8] Two-dimensional gas-phase temperature measurements using fluorescence lifetime imaging [J].

Ni, TQ ;

Melton, LA .

APPLIED SPECTROSCOPY, 1996, 50 (09) :1112-1116

[9] AGING OF PROTEINS - ISOLATION AND IDENTIFICATION OF A FLUORESCENT CHROMOPHORE FROM THE REACTION OF POLYPEPTIDES WITH GLUCOSE [J].

PONGOR, S ;

ULRICH, PC ;

BENCSATH, FA ;

CERAMI, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (09) :2684-2688

[10] QUANTITATIVE PH IMAGING IN CELLS USING CONFOCAL FLUORESCENCE LIFETIME IMAGING MICROSCOPY [J].

SANDERS, R ;

DRAAIJER, A ;

GERRITSEN, HC ;

HOUPT, PM ;

LEVINE, YK .

ANALYTICAL BIOCHEMISTRY, 1995, 227 (02) :302-308