Detection of Escherichia coli O157:H7 from Musca domestica (Diptera: Muscidae) at a cattle farm in Japan

被引：89

作者：

Iwasa, M ^{[1
]}

Makino, S

Asakura, H

Kobori, H

Morimoto, Y

机构：

[1] Obihiro Univ Agr & Vet Med, Entomol Lab, Obihiro, Hokkaido 080, Japan

[2] Obihiro Univ Agr & Vet Med, Dept Vet Microbiol, Obihiro, Hokkaido 080, Japan

[3] Hokkaido Inst Publ Hlth, Bacteriol Sect, Sapporo, Hokkaido, Japan

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 1999年 / 36卷 / 01期

关键词：

enterohemorrhagic Escherichia coli O157 : H7; Musca domestica; Shiga-toxin; polymerase chain reaction;

D O I：

10.1093/jmedent/36.1.108

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 was isolated for the first time from Musca domestica L. A total of 310 ny samples was collected from 4 different farms in Obihiro-City, Hokkaido, in the summer and autumn of 1997; 5 samples carried E. coli serotype O157:H7. Using ELISA and Vero cell cytotoxicity assays, 3 isolates from 1 cattle farm produced both active Shiga-toxin type 1 (Stx1) and 2 (Stx2). These isolates also carried hemolysin and eaeA genes and harbored the SO-kb virulence plasmid of EHEC O157:H7. Based on plasmid profiles, antibiotic patterns, polymerase chain reaction (PCR)-based DNA finger printing analysis using random amplified polymorphic DNA, pulsed field gel electrophoresis analysis, and DNA sequences of stx(1) and stx(2), all 3 isolates from ny samples were identical. These results indicate that the house ny is capable of carrying the toxigenic EHEC O157:H7 involved in human disease.

引用

页码：108 / 112

页数：5

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