Cell cycle-dependent recruitment of HDAC-1 correlates with deacetylation of histone H4 on an Rb-E2F target promoter

被引:86
作者
Ferreira, R [1 ]
Naguibneva, I [1 ]
Mathieu, M [1 ]
Ait-Si-Ali, S [1 ]
Robin, P [1 ]
Pritchard, LL [1 ]
Harel-Bellan, A [1 ]
机构
[1] Inst Federat Andre Lwoff, CNRS UPR 9079, Lab Oncogenese Differenciat & Transduct Signal, F-94801 Villejuif, France
关键词
D O I
10.1093/embo-reports/kve173
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transcription factor E2F, which is a key element in the control of cell proliferation, is repressed by Rb and other pocket proteins in growth-arrested differentiating cells, as well as in proliferating cells when they progress through early G(1). It is not known whether similar mechanisms are operative in the two situations. A body of data suggests that E2F repression by pocket proteins involves class I histone deacetylases (HDACs). It has been hypothesized that these enzymes are recruited to E2F target promoters where they deacetylate histones. Here we have tested this hypothesis directly by using formaldehyde cross-linked chromatin immunoprecipitation (XChIP) assays to evaluate HDAC association in living cells. Our data show that a histone deacetylase, HDAC-1, is stably bound to an E2F target promoter during early G, in proliferating cells and released at the G(1)-S transition. In addition, our results reveal an inverse correlation between HDAC-1 recruitment and histone H4 acetylation on specific lysines.
引用
收藏
页码:794 / 799
页数:6
相关论文
共 27 条
[1]  
Brehm A, 1999, BRIT J CANCER, V80, P38
[2]   Retinoblastoma protein recruits histone deacetylase to repress transcription [J].
Brehm, A ;
Miska, EA ;
McCance, DJ ;
Reid, JL ;
Bannister, AJ ;
Kouzarides, T .
NATURE, 1998, 391 (6667) :597-601
[3]  
DEGREGORI J, 1995, MOL CELL BIOL, V15, P4215
[4]   The three members of the pocket proteins family share the ability to regress E2F activity through recruitment of a histone deacetylase [J].
Ferreira, R ;
Magnaghi-Jaulin, L ;
Robin, P ;
Harel-Bellan, A ;
Trouche, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10493-10498
[5]   Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain [J].
Fry, CJ ;
Slansky, JE ;
Farnham, PJ .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (04) :1966-1976
[6]  
Geng Y, 1996, ONCOGENE, V12, P1173
[7]   A role for histone deacetylase activity in HDAC1-mediated transcriptional repression [J].
Hassig, CA ;
Tong, JK ;
Fleischer, TC ;
Owa, T ;
Grable, PG ;
Ayer, DE ;
Schreiber, SL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3519-3524
[8]   Acetylation: a regulatory modification to rival phosphorylation? [J].
Kouzarides, T .
EMBO JOURNAL, 2000, 19 (06) :1176-1179
[9]  
Laj A, 1999, MOL CELL BIOL, V19, P6632
[10]   AN E2F-BINDING SITE MEDIATES CELL-CYCLE-REGULATED REPRESSION OF MOUSE B-MYB TRANSCRIPTION [J].
LAM, EWF ;
WATSON, RJ .
EMBO JOURNAL, 1993, 12 (07) :2705-2713