Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices

被引:42
作者
Fuentes, Hernan V. [1 ]
Woolley, Adam T. [1 ]
机构
[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
关键词
D O I
10.1039/b708865e
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Electrolysis-based micropumps integrated with microfluidic channels in micromachined glass substrates are presented. Photolithography combined with wet chemical etching and thermal bonding enabled the fabrication of multi-layer devices containing electrically actuated micropumps interfaced with sample and mobile phase reservoirs. A stationary phase was deposited on the microchannel walls by coating with 10% (w/w) chlorodimethyloctadecylsilane in toluene. Pressure- balanced injection was implemented by controlling the electrolysis time and voltage applied in the two independent micropumps. Current fluctuations in the micropumps due to the stochastic formation of bubbles on the electrode surfaces were determined to be the main cause of variation between separations. On-chip electrochemical pumping enabled the loading of pL samples with no dead volume between injection and separation. A mobile phase composed of 70% acetonitrile and 30% 50 mM acetate buffer (pH 5.45) was used for the chromatographic separation of three fluorescently labeled amino acids in,40 s with an efficiency of 3000 theoretical plates in a 2.5 cm-long channel. Our results demonstrate the potential of electrochemical micropumps integrated with microchannels to perform rapid chromatographic separations in a microfabricated platform. Importantly, these devices represent a significant step toward the development of miniaturized and fully integrated liquid chromatography systems.
引用
收藏
页码:1524 / 1531
页数:8
相关论文
共 59 条
[1]  
BARDELL R, 1997, MICROELECTROMECHANIC, V354, P47
[2]   A closed-loop controlled electrochemically actuated micro-dosing system [J].
Böhm, S ;
Timmer, B ;
Olthuis, W ;
Bergveld, P .
JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2000, 10 (04) :498-504
[3]   Trace-level amino acid analysis by capillary liquid chromatography and application to in vivo microdialysis sampling with 10-s temporal resolution [J].
Boyd, BW ;
Witowski, SR ;
Kennedy, RT .
ANALYTICAL CHEMISTRY, 2000, 72 (04) :865-871
[4]   Electrolytic actuators: Alternative, high-performance, material-based devices [J].
Cameron, CG ;
Freund, MS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7827-7831
[5]   A PIEZOELECTRIC-DRIVEN STEREOLITHOGRAPHY-FABRICATED MICROPUMP [J].
CARROZZA, MC ;
CROCE, N ;
MAGNANI, B ;
DARIO, P .
JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 1995, 5 (02) :177-179
[6]   Instrumental requirements for nanoscale liquid chromatography [J].
Chervet, JP ;
Ursem, M ;
Salzmann, JB .
ANALYTICAL CHEMISTRY, 1996, 68 (09) :1507-1512
[7]   A Microfabricated Rotary Pump [J].
Chou, Hou-Pu ;
Unger, Marc A. ;
Quake, Stephen R. .
BIOMEDICAL MICRODEVICES, 2001, 3 (04) :323-330
[8]   On-chip chromatography: the last twenty years [J].
de Mello, A .
LAB ON A CHIP, 2002, 2 (03) :48N-54N
[9]   Micro total analysis systems. Latest advancements and trends [J].
Dittrich, Petra S. ;
Tachikawa, Kaoru ;
Manz, Andreas .
ANALYTICAL CHEMISTRY, 2006, 78 (12) :3887-3907
[10]   Dispersion reduction in open-channel liquid electrochromatographic columns via pressure-driven back flow [J].
Dutta, D ;
Leighton, DT .
ANALYTICAL CHEMISTRY, 2003, 75 (14) :3352-3359