Release of telomeric DNA from chromosomes in immortal human cells lacking telomerase activity

被引：73

作者：

Ogino, H

Nakabayashi, K

Suzuki, M

Takahashi, E

Fujii, M

Suzuki, T

Ayusawa, D

机构：

[1] Yokohama City Univ, Kihara Inst Biol Res, Div Biochem, Totsuka Ku, Yokohama, Kanagawa 2440813, Japan

[2] Yokohama City Univ, Grad Sch Integrated Sci, Totsuka Ku, Yokohama, Kanagawa 2440813, Japan

[3] Otsuka Pharmaceut Co Ltd, Otsuka GEN Res Inst, Tokushima 77101, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.8875

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Some immortal human cell lines lack telomerase activity. These cell lines were found to contain small dispersed DNA hybridizing to TTAGGG: repeats. Such DNA was located in their cytoplasm and nuclei, Normal human fibroblasts or telomerase-positive cell lines did not contain such DNA. Upon cloning and sequencing, it was shown to consist of TTAGGG repeats. When electrophoresed on neutral and alkaline agarose gels, it behaved as double-stranded and linear DNA. These results suggest that telomeric DNA is released from chromosomes in association with maintenance of telomeres in telomerase-negative cell lines. (C) 1998 Academic Press.

引用

页码：223 / 227

页数：5

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