Amperometric Quantification of Gluten in Food Samples Using an ELISA Competitive Assay and Flow Injection Analysis

被引：16

作者：

Amaya-Gonzalez, Sonia ^{[1
]}

de-los-Santos-Alvarez, Noemi ^{[1
]}

Jesus Lobo-Castanon, Maria ^{[1
]}

Miranda-Ordieres, Arturo J. ^{[1
]}

Tunon-Blanco, Paulino ^{[1
]}

机构：

[1] Univ Oviedo, Dept Quim Fis & Analit, E-33006 Oviedo, Spain

来源：

ELECTROANALYSIS | 2011年 / 23卷 / 01期

关键词：

33-mer peptide; ELISA competitive assay; Electrochemical detection; Gluten; Gliadin; DISEASE TOXIC GLIADIN; CELIAC-DISEASE; IMMUNOSENSOR; IMMUNOASSAY; PEPTIDE;

D O I：

10.1002/elan.201000583

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Gluten analysis in food is intricate because of the analyte variability among cereal species and protein states. Herein the immunodominant peptide 33-mer from alpha2-gliadin is proposed as standard for calibration in a competitive ELISA assay with amperometric flow-injection detection. Using the monoclonal antibody G12, a correlation between the content of gluten in peptide and gluten units was obtained for the first time. The analytical features were compared with optical measurements using several standards obtaining a slightly lower detection limit, which suggested the sensitivity is limited by affinity and not by the detection technique. "Gluten-free" samples were satisfactorily analyzed.

引用

页码：108 / 114

页数：7

共 14 条

[1]

[Anonymous], RIDASCREEN GLIADIN C

[2]

Codex Alimentarius Commission, 2006, 27 SESS COD COMM MET

[3]

Development of a novel electrochemical immuno-assay using a screen printed electrode for the determination of secretory immunoglobulin A in human sweat [J].

Crew, A. ;

Alford, C. ;

Cowell, D. C. C. ;

Hart, J. P. .

ELECTROCHIMICA ACTA, 2007, 52 (16) :5232-5237

[4]

Measurement of gluten using a monoclonal antibody to a coeliac toxic peptide of A gliadin [J].

Ellis, HJ ;

Rosen-Bronson, S ;

O'Reilly, N ;

Ciclitira, PJ .

GUT, 1998, 43 (02) :190-195

[5]

Increasing prevalence of coeliac disease over time [J].

Lohi, S. ;

Mustalahti, K. ;

Kaukinen, K. ;

Laurila, K. ;

Collin, P. ;

Rissanen, H. ;

Lohi, O. ;

Bravi, E. ;

Gasparin, M. ;

Reunanen, A. ;

Maeki, M. .

ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2007, 26 (09) :1217-1225

[6]

Sensitive detection of cereal fractions that are toxic to celiac disease patients by using monoclonal antibodies to a main immunogenic wheat peptide [J].

Moron, Beln ;

Cebolla, Angel ;

Manyani, Hamid ;

Alvarez-Maqueda, Moises ;

Megias, Manuel ;

Thomas, Maria del Carmen ;

Lopez, Manuel Carlos ;

Sousa, Carolina .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 2008, 87 (02) :405-414

[7]

Electrochemical Immunosensor for Detection of Celiac Disease Toxic Gliadin in Foodstuff [J].

Nassef, Hossam M. ;

Redondo, M. Carmen Bermudo ;

Ciclitira, Paul J. ;

Ellis, H. Julia ;

Fragoso, Alex ;

O'Sullivan, Ciara K. .

ANALYTICAL CHEMISTRY, 2008, 80 (23) :9265-9271

[8]

Amperometric Immunosensor for Detection of Celiac Disease Toxic Gliadin Based on Fab Fragments [J].

Nassef, Hossam M. ;

Civit, Laia ;

Fragoso, Alex ;

O'Sullivan, Ciara K. .

ANALYTICAL CHEMISTRY, 2009, 81 (13) :5299-5307

[9]

Homogeneous stopped-flow fluorimmunoassay for gliadin determination in food samples [J].

Sánchez-Martínez, ML ;

Aguilar-Caballos, MP ;

Gómez-Hens, A .

ANALYTICA CHIMICA ACTA, 2004, 523 (01) :35-41

[10]

SKERRITT JH, 1991, J ASSOC OFF ANA CHEM, V74, P257

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