N-acetylcysteine enhances endothelium-dependent vasorelaxation in the isolated rat mesenteric artery

被引:28
作者
Lopez, BL [1 ]
Snyder, JW [1 ]
Birenbaum, DS [1 ]
Ma, XL [1 ]
机构
[1] Thomas Jefferson Univ, Jefferson Med Coll, Div Emergency Med, Dept Surg, Philadelphia, PA 19107 USA
关键词
D O I
10.1016/S0196-0644(98)70167-2
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Study hypothesis: Previous studies have suggested that N-acetylcysteine (NAC) may confer additional protection in acetaminophen (APAP) overdose by improving hepatic microcirculation. We hypothesize that NAC enhances release of nitric oxide (NO) from the vasculature. Methods: Sprague-Dawley rat superior mesenteric artery rings were suspended in oxygenated Krebs-Henseleit tissue baths and contracted with U-46619 (a thromboxane A(2)-mimetic). In part 1, the effect of NAC on endothelial cell (EC) release of NO was assessed by measurement of vasorelaxation induced by acetylcholine (ACh, an EC-dependent vasorelaxor) in the presence and absence of NAG. In part 2, the effect of glutathione (a major component of NAC hepatoprotection) was examined by measuring ACh-induced vasorelaxation in rings from rat treated with L-buthionine sulfoxamine (BSO, a glutathione synthesis inhibitor). Data were analyzed by repeated-measures ANOVA. Results: Addition of 15 to 30 mmol/l NAC after ring contraction had no direct vasodilatory effect. By contrast, pretreatment of rings with NAC(15 mmol/L) enhanced vasorelaxation induced by ACh (95.0%+/-7.9% versus 62.3%+/-7.6% for control; ACh dose, 1 mu mol/L; P<.001) or by A23187, a receptor-independent, NO-mediated vasodilator (91.6%+/-9.6% versus 68.3%+/-12.1% for control; A23187 dose, 1 mu mol/L; P<.001). In rings from BSO-treated rats, NAC also enhanced vasorelaxation (76.5%+/-7.1%; P<.001 versus control), but to a lesser degree than in nontreated rats. Conclusion: NAC enhances endothelium-dependent vasodilation in an isolated rat mesenteric artery ring preparation. In addition to its antioxidant effects, NAC may decrease APAP hepatotoxicity by stimulating NO production and improving microvascular circulation.
引用
收藏
页码:405 / 410
页数:6
相关论文
共 31 条
[1]   NO+, NO(CENTER-DOT), AND NO- DONATION BY S-NITROSOTHIOLS - IMPLICATIONS FOR REGULATION OF PHYSIOLOGICAL FUNCTIONS BY S-NITROSYLATION AND ACCELERATION OF DISULFIDE FORMATION [J].
ARNELLE, DR ;
STAMLER, JS .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 318 (02) :279-285
[2]   NITRIC-OXIDE AND CARDIOVASCULAR CONTROL [J].
CALVER, A ;
COLLIER, J ;
VALLANCE, P .
EXPERIMENTAL PHYSIOLOGY, 1993, 78 (03) :303-326
[3]   INACTIVATION OF ENDOTHELIAL DERIVED RELAXING FACTOR BY OXIDIZED LIPOPROTEINS [J].
CHIN, JH ;
AZHAR, S ;
HOFFMAN, BB .
JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (01) :10-18
[4]   Effects of a hydroxylated metabolite of the β-adrenoreceptor antagonist, carvedilol, on post-ischaemic splanchnic tissue injury [J].
Christopher, TA ;
Lopez, BL ;
Ma, XL ;
Feuerstein, GZ ;
Ruffolo, RR ;
Yue, TL .
BRITISH JOURNAL OF PHARMACOLOGY, 1998, 123 (02) :292-298
[5]   REVERSIBLE AND IRREVERSIBLE INHIBITION OF HEPATIC MITOCHONDRIAL RESPIRATION BY ACETAMINOPHEN AND ITS TOXIC METABOLITE, N-ACETYL-PARA-BENZOQUINONEIMINE (NAPQI) [J].
ESTERLINE, RL ;
RAY, SD ;
JI, SC .
BIOCHEMICAL PHARMACOLOGY, 1989, 38 (14) :2387-2390
[6]   THE OBLIGATORY ROLE OF ENDOTHELIAL-CELLS IN THE RELAXATION OF ARTERIAL SMOOTH-MUSCLE BY ACETYLCHOLINE [J].
FURCHGOTT, RF ;
ZAWADZKI, JV .
NATURE, 1980, 288 (5789) :373-376
[7]   IMPROVED OUTCOME OF PARACETAMOL-INDUCED FULMINANT HEPATIC-FAILURE BY LATE ADMINISTRATION OF ACETYLCYSTEINE [J].
HARRISON, PM ;
KEAYS, R ;
BRAY, GP ;
ALEXANDER, GJM ;
WILLIAMS, R .
LANCET, 1990, 335 (8705) :1572-1573
[8]   IMPROVEMENT BY ACETYLCYSTEINE OF HEMODYNAMICS AND OXYGEN-TRANSPORT IN FULMINANT HEPATIC-FAILURE [J].
HARRISON, PM ;
WENDON, JA ;
GIMSON, AES ;
ALEXANDER, GJM ;
WILLIAMS, R .
NEW ENGLAND JOURNAL OF MEDICINE, 1991, 324 (26) :1852-1857
[9]   NITRIC-OXIDE - A CYTO-TOXIC ACTIVATED MACROPHAGE EFFECTOR MOLECULE [J].
HIBBS, JB ;
TAINTOR, RR ;
VAVRIN, Z ;
RACHLIN, EM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (01) :87-94
[10]   ENDOTHELIUM-DERIVED RELAXING FACTOR PRODUCED AND RELEASED FROM ARTERY AND VEIN IS NITRIC-OXIDE [J].
IGNARRO, LJ ;
BUGA, GM ;
WOOD, KS ;
BYRNS, RE ;
CHAUDHURI, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :9265-9269