The primordial high energy compound: ATP or inorganic pyrophosphate?

被引：52

作者：

Chi, A ^{[1
]}

Kemp, RG ^{[1
]}

机构：

[1] Chicago Med Sch, Dept Biochem & Mol Biol, N Chicago, IL 60064 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 46期

关键词：

D O I：

10.1074/jbc.C000581200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pyrophosphate-dependent phosphofructokinase (PPi-PFK) of Entamoeba histolytica displays a millionfold preference for inorganic pyrophosphate (PP,) over ATP (calculated as the ratio of k(cat)/K-m). The introduction of a single mutation by site-directed mutagenesis changes its preference from PP, to ATP. The single mutant has an 8-fold preference for ATP whereas a related double mutant shows a preference exceeding 10,000-fold. The results suggest the presence of a latent nucleotide binding site aligned for a catalytic role in PPi-PFK. It is proposed that the ancestral PFK was an ATP-dependent enzyme and that PPi-PFKs are a later evolving adaptation.

引用

页码：35677 / 35679

页数：3

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