Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression

被引：18

作者：

Li, Xihai ^{[1
]}

Chen, Jiashou ^{[1
]}

Liang, Wenna ^{[2
]}

Li, Huiting ^{[3
]}

Liu, Fayuan ^{[1
]}

Weng, Xiaping ^{[1
]}

Lin, Pingdong ^{[3
]}

Chen, Wenlie ^{[1
]}

Zheng, Chunsong ^{[1
]}

Xu, Huifeng ^{[1
]}

Liu, Xianxiang ^{[1
]}

Ye, Hongzhi ^{[1
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350122, Fujian, Peoples R China

[2] Fujian Univ Tradit Chinese Med, Res Base Tradit Chinese Med Syndrome, Fuzhou 350122, Fujian, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Fujian Key Lab Integrat Med Geriatr, Fuzhou 350122, Fujian, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2015年 / 9卷 / 03期

关键词：

chondrocyte; cell cycle; osteoarthritis; Bushen Zhuangjin Decoction; KNEE OSTEOARTHRITIS; ARTICULAR CHONDROCYTES; EXPRESSION; TRANSITION; EXPANSION; HIP;

D O I：

10.3892/etm.2015.2214

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Bushen Zhuangjin Decoction (BZD), a well-known formulation in Traditional Chinese Medicine, has been widely used for the treatment of osteoarthritis (OA). Due to the poor intrinsic repair capacity of chondrocytes, promoting the proliferation of chondrocytes is an efficient treatment to delay the progression of cartilage degradation. The present study, therefore, focused on the effect of BZD on chondrocyte proliferation, exploring the mechanism of BZD on the inhibition of cartilage degradation. Chondrocytes isolated from the knee articular cartilage of Sprague Dawley rats were cultured and identified by type II collagen immunohistochemistry. It was found that BZD promoted chondrocyte viability in a dose- and time-dependent manner. To investigate if BZD promoted the chondrocyte viability by stimulating the cell cycle progression a flow cytometer was used, and the results showed that the percentage proportion of G0/G1 cells was significantly lower, and the percentage proportion of S cells was significantly higher, in treated cells compared with that in untreated cells. To gain insight into the mechanism underlying the effect of BZD on the cell cycle progression, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4), CDK6 and p21 was measured by reverse transcription-polymerase chain reaction and western blotting, respectively. The mRNA and protein expression of cyclin D1, CDK4 and CDK6 in the BZD-treated chondrocytes was significantly upregulated, while the mRNA and protein expression of p21 was significantly downregulated, compared with that in the untreated chondrocytes. These results suggested that BZD promoted chondrocyte proliferation by accelerating G1/S transition, indicating that BZD is a potential therapeutic agent for the treatment of OA.

引用

页码：839 / 844

页数：6

共 24 条

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