The receptors for mammalian sweet and umami taste

被引:981
作者
Zhao, GQ
Zhang, YF
Hoon, MA
Chandrashekar, J
Erlenbach, I
Ryba, NJP
Zuker, CS
机构
[1] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA
[4] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA
关键词
METABOTROPIC GLUTAMATE-RECEPTOR; PHOSPHOLIPASE-C; GENE-EXPRESSION; CHORDA TYMPANI; BITTER TASTE; RESPONSES; CELLS; MICE; IDENTIFICATION; FAMILY;
D O I
10.1016/S0092-8674(03)00844-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sweet and umami (the taste of monosodium glutamate) are the main attractive taste modalities in humans. T1Rs are candidate mammalian taste receptors that combine to assemble two heteromeric G-protein-coupled receptor complexes: T1R1+3, an umami sensor, and T1R2+3, a sweet receptor. We now report the behavioral and physiological characterization of T1R1, T1R2, and T1R3 knockout mice. We demonstrate that sweet and umami taste are strictly dependent on T1R-receptors, and show that selective elimination of T1R-subunits differentially abolishes detection and perception of these two taste modalities. To examine the basis of sweet tastant recognition and coding, we engineered animals expressing either the human T1R2-receptor (hT1R2), or a modified opioid-receptor (RASSL) in sweet cells. Expression of hT1R2 in mice generates animals with humanized sweet taste preferences, while expression of RASSL drives strong attraction to a synthetic opiate, demonstrating that sweet cells trigger dedicated behavioral outputs, but their tastant selectivity is determined by the nature of the receptors.
引用
收藏
页码:255 / 266
页数:12
相关论文
共 40 条
[1]   Adenylyl cyclase expression and modulation of cAMP in rat taste cells [J].
Abaffy, T ;
Trubey, KR ;
Chaudhari, N .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2003, 284 (06) :C1420-C1428
[2]   A novel family of mammalian taste receptors [J].
Adler, E ;
Hoon, MA ;
Mueller, KL ;
Chandrashekar, J ;
Ryba, NJP ;
Zuker, CS .
CELL, 2000, 100 (06) :693-702
[3]   Intake of umami-tasting solutions by mice: A genetic analysis [J].
Bachmanov, AA ;
Tordoff, MG ;
Beauchamp, GK .
JOURNAL OF NUTRITION, 2000, 130 (04) :935S-941S
[4]   The human TAS2R16 receptor mediates bitter taste in response to β-glucopyranosides [J].
Bufe, B ;
Hofmann, T ;
Krautwurst, D ;
Raguse, JD ;
Meyerhof, W .
NATURE GENETICS, 2002, 32 (03) :397-401
[5]   T2Rs function as bitter taste receptors [J].
Chandrashekar, J ;
Mueller, KL ;
Hoon, MA ;
Adler, E ;
Feng, LX ;
Guo, W ;
Zuker, CS ;
Ryba, NJP .
CELL, 2000, 100 (06) :703-711
[6]   Molecular and physiological evidence for glutamate (Umami) taste transduction via a G protein-coupled receptor [J].
Chaudhari, N ;
Roper, SD .
OLFACTION AND TASTE XII: AN INTERNATIONAL SYMPOSIUM, 1998, 855 :398-406
[7]   A metabotropic glutamate receptor variant functions as a taste receptor [J].
Chaudhari, N ;
Landin, MA ;
Roper, SD .
NATURE NEUROSCIENCE, 2000, 3 (02) :113-119
[8]  
Chaudhari N, 1996, J NEUROSCI, V16, P3817
[9]   Neural responses to bitter compounds in rats [J].
Dahl, M ;
Erickson, RP ;
Simon, SA .
BRAIN RESEARCH, 1997, 756 (1-2) :22-34
[10]   Detection of sweet and umami taste in the absence of taste receptor T1r3 [J].
Damak, S ;
Rong, MQ ;
Yasumatsu, K ;
Kokrashvili, Z ;
Varadarajan, V ;
Zou, SY ;
Jiang, PH ;
Ninomiya, Y ;
Margolskee, RF .
SCIENCE, 2003, 301 (5634) :850-853