The IaIA invasion gene of Bartonella bacilliformis encodes a (di)nucleoside polyphosphate hydrolase of the MutT motif family and has homologs in other invasive bacteria

被引:68
作者
Cartwright, JL
Britton, P
Minnick, MF
McLennan, AG
机构
[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England
[2] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA
关键词
D O I
10.1006/bbrc.1999.0354
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The product of the ialA invasion gene of Bartonella bacilliformis has been expressed as a thioredoxin fusion protein, it is a (di)nucleoside polyphosphate hydrolase of the MutT motif protein family with strong sequence similarity to plant diadenosine tetraphosphate hydrolases. It hydrolyses nucleoside and dinucleoside polyphosphates with four or more phosphate groups, always producing an NTP as one product. Diadenosine tetraphosphate (Ap(4)A) is the preferred substrate with a K-m of 10 mu M and a k(cat) of 3.0 s(-1). It is inhibited by Ca2+ and F- (K-i = 80 mu M). Hydrolysis of Ap(4)A in (H2O)-O-18 yielded [O-18]AMP as the only labelled product. in terms of sequence, reaction mechanism and properties, IalA is very similar to eukaryotic Ap(4)A hydrolases, and unlike previously described bacterial Ap(4)A hydrolases.: Homologs are present in the genomes of other invasive pathogens. They may function: to reduce stress-induced dinucleotide levels during invasion and so enhance pathogen survival. (C) 1999 Academic Press.
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页码:474 / 479
页数:6
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