Biochemical and biomedical application of monodisperse polymer particles

被引:50
作者
Ugelstad, J
Stenstad, P
Kilaas, L
Prestvik, WS
Rian, A
Nustad, K
Herje, R
Berge, A
机构
[1] UNIV TRONDHEIM, DEPT IND CHEM, N-7034 TRONDHEIM, NORWAY
[2] NORWEGIAN RADIUM HOSP, CENT LAB, N-0310 OSLO, NORWAY
关键词
D O I
10.1002/masy.19961010155
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Macroporous particles with large pores are used for size exclusion chromatography of carbohydrates with very high molecular weights. Macroporous particles of different sizes are used for multiple immunofluorometric analysis by flow cytometry. Porous magnetic particles with protein A coupled to the surface of the pores combine a high capacity in binding of IgG with easy handling of the particles. Magnetic beads with boronic acid coupled to the surface are applied for selective isolation of subclasses of cells. The beads are detached from the cells by addition of sorbitol, leaving free, pure cell fractions in good yield. The use of magnetic particles in molecular biology is described.
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页码:491 / 500
页数:10
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