Endocytic properties of the M-type 180-kDa receptor for secretory phospholipases A(2)

被引:113
作者
Zvaritch, E [1 ]
Lambeau, G [1 ]
Lazdunski, P [1 ]
机构
[1] INST PHARMACOL MOLEC & CELLULAIRE,F-06560 VALBONNE,FRANCE
关键词
D O I
10.1074/jbc.271.1.250
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Endocytic properties of the M-type 180-kDa receptor for secretory phospholipases A(2)(sPLA(2)) were first investigated in rabbit myocytes that express it at high levels. Internalization of the receptor was shown to be clathrin-coated pit-mediated, rapid (k(e) = 0.1 min(-1)), and ligand-independent. The signal sequence for internalization was then identified upon transient and stable expression of various receptor constructs with mutated cytoplasmic sequences. Analysis of the internalization efficiency of the mutants suggested that the NSYY motif encodes the major endocytic signal, with the distal tyrosine residue playing the key role. Amino acid substitutions at the putative casein kinase II phosphorylation site of the receptor did not affect internalization. A chimeric protein composed of the extracellular and transmembrane domains of the rabbit sPLA(2) receptor and of the cytoplasmic domain of the structurally homologous human macrophage mannose receptor retained the high affinity for sPLA(2) and was internalization competent, exhibiting 50% endocytic activity of the M-type sPLA(2) receptor, The results indicate the compatibility of the structural domains of the two parent proteins and provide evidence for the interchangeable character of their internalization signals.
引用
收藏
页码:250 / 257
页数:8
相关论文
共 69 条
[1]   NEF INDUCES CD4 ENDOCYTOSIS - REQUIREMENT FOR A CRITICAL DILEUCINE MOTIF IN THE MEMBRANE-PROXIMAL CD4 CYTOPLASMIC DOMAIN [J].
AIKEN, C ;
KONNER, J ;
LANDAU, NR ;
LENBURG, ME ;
TRONO, D .
CELL, 1994, 76 (05) :853-864
[2]  
ALMOND BD, 1994, J BIOL CHEM, V269, P26635
[3]   THE HUMAN 180-KDA RECEPTOR FOR SECRETORY PHOSPHOLIPASES A(2) - MOLECULAR-CLONING, IDENTIFICATION OF A SECRETED SOLUBLE FORM, EXPRESSION, AND LOCALIZATION [J].
ANCIAN, P ;
LAMBEAU, G ;
MATTEI, MG ;
LAZDUNSKI, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8963-8970
[4]  
ARITA H, 1991, J BIOL CHEM, V266, P19139
[5]   THE INSULIN-RECEPTOR JUXTAMEMBRANE REGION CONTAINS 2 INDEPENDENT TYROSINE BETA-TURN INTERNALIZATION SIGNALS [J].
BACKER, JM ;
SHOELSON, SE ;
WEISS, MA ;
HUA, QX ;
CHEATHAM, RB ;
HARING, E ;
CAHILL, DC ;
WHITE, MF .
JOURNAL OF CELL BIOLOGY, 1992, 118 (04) :831-839
[6]   THE NPXY INTERNALIZATION SIGNAL OF THE LDL RECEPTOR ADOPTS A REVERSE-TURN CONFORMATION [J].
BANSAL, A ;
GIERASCH, LM .
CELL, 1991, 67 (06) :1195-1201
[7]   MOLECULAR AND CELLULAR BIOLOGY OF INSULIN-RECEPTOR INTERNALIZATION [J].
CARPENTIER, JL ;
PACCAUD, JP .
MOLECULAR AND CELL BIOLOGICAL ASPECTS OF GASTROENTEROPANCREATIC NEUROENDOCRINE TUMOR DISEASE, 1994, 733 :266-278
[8]  
CHANG CP, 1993, J BIOL CHEM, V268, P19312
[9]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[10]  
CHEN HJ, 1993, J BIOL CHEM, V268, P22338