Chitosan/siRNA Nanoparticles Biofunctionalize Nerve Implants and Enable Neurite Outgrowth

被引：64

作者：

Mittnacht, Ursula ^{[1
]}

Hartmann, Hanna ^{[1
]}

Hein, San ^{[2
,3
]}

Oliveira, Hugo ^{[4
,5
]}

Dong, Mingdong ^{[2
,3
]}

Pego, Ana P. ^{[4
]}

Kjems, Jorgen ^{[2
,3
]}

Howard, Kenneth A. ^{[2
,3
]}

Schlosshauer, Burkhard ^{[1
]}

机构：

[1] Univ Tubingen, NMI Nat & Med Sci Inst, D-72770 Reutlingen, Germany

[2] Univ Aarhus, Interdisciplinary Nanosci Ctr, DK-8000 Aarhus C, Denmark

[3] Univ Aarhus, Dept Mol Biol, DK-8000 Aarhus C, Denmark

[4] Univ Porto, INEB, Div Biomat, P-4150180 Oporto, Portugal

[5] Univ Porto, Fac Engn, P-4200465 Oporto, Portugal

来源：

NANO LETTERS | 2010年 / 10卷 / 10期

关键词：

Nanoparucles; Si RNA; chitosan; nerve regeneration; nanobiofunctionalized implants; drug delivery; MYELIN-ASSOCIATED GLYCOPROTEIN; RECEPTOR FAMILY-MEMBER; NONVIRAL GENE DELIVERY; RNA INTERFERENCE; NOGO RECEPTOR; AXONAL REGENERATION; IN-VITRO; SIRNA; INHIBITOR; TRANSFECTION;

D O I：

10.1021/nl1016909

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Microstructured 2 0 Aim thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparucles to promote nerve regeneration and ensure local delivery of nanotherapeutics The stable nanoparticles were rapidly internalized by cells and did not affect cell viability Target mRNA was successfully reduced by 65-75% and neurite outgrowth was enhanced even in an inhibitory environment This work, thus. supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair

引用

页码：3933 / 3939

页数：7

共 49 条

[1]

Disinhibition of neurotrophin-induced dorsal root ganglion cell neurite outgrowth on CNS myelin by siRNA-mediated knockdown of NgR, p75NTR and Rho-A [J].

Ahmed, Z ;

Dent, RG ;

Suggate, EL ;

Barrett, LB ;

Seabright, RJ ;

Berry, M ;

Logan, A .

MOLECULAR AND CELLULAR NEUROSCIENCE, 2005, 28 (03) :509-523

[2]

Physical stabilization of DNA-based therapeutics [J].

Anchordoquy, TJ ;

Allison, SD ;

Molina, MDC ;

Girouard, LG ;

Carson, TK .

DRUG DISCOVERY TODAY, 2001, 6 (09) :463-470

[3]

Delivery of siRNA from lyophilized polymeric surfaces [J].

Andersen, Morten O. ;

Howard, Kenneth A. ;

Paludan, Soren R. ;

Besenbacher, Flemming ;

Kjems, Jorgen .

BIOMATERIALS, 2008, 29 (04) :506-512

[4]

Tissue-engineered autologous bladders for patients needing cystoplasty [J].

Atala, A ;

Bauer, SB ;

Soker, S ;

Yoo, JJ ;

Retik, AB .

LANCET, 2006, 367 (9518) :1241-1246

[5]

Inhibition of respiratory viruses by nasally administered siRNA [J].

Bitko, V ;

Musiyenko, A ;

Shulyayeva, O ;

Barik, S .

NATURE MEDICINE, 2005, 11 (01) :50-55

[6]

Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1 [J].

Chen, MS ;

Huber, AB ;

van der Haar, ME ;

Frank, M ;

Schnell, L ;

Spillmann, AA ;

Christ, F ;

Schwab, ME .

NATURE, 2000, 403 (6768) :434-439

[7]

Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles [J].

Corsi, K ;

Chellat, F ;

Yahia, L ;

Fernandes, JC .

BIOMATERIALS, 2003, 24 (07) :1255-1264

[8]

Interfering with disease: a progress report on siRNA-based therapeutics [J].

de Fougerolles, Antonin ;

Vornlocher, Hans-Peter ;

Maraganore, John ;

Lieberman, Judy .

NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (06) :443-453

[9]

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[10]

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

← 1 2 3 4 5 →