HPLC-Q-TOF-MS Identification of Antioxidant and Antihypertensive Peptides Recovered from Cherry (Prunus cerasus L.) Subproducts

被引：57

作者：

Concepcion Garcia, Maria ^{[1
]}

Endermann, Jochan ^{[1
]}

Gonzalez-Garcia, Estefania ^{[1
]}

Luisa Marina, Maria ^{[1
]}

机构：

[1] Univ Alcala, Dept Analyt Chem, E-28871 Madrid, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 05期

关键词：

cherry; seed proteins; peptide; antihypertensive; antioxidant; MS identification; PERFORMANCE; EXTRACTION; OXIDATION; STRATEGY; STONE;

D O I：

10.1021/jf505037p

中图分类号：

S [农业科学];

学科分类号：

082806 [农业信息与电气工程];

摘要：

The processing of fruits, such as cherries, is characterized by generating a lot of waste material such as fruit stones, skins, etc. To contribute to environmental sustainability, it is necessary to recover these residues. Cherry stones contain seeds with a significant amount of proteins that are underused and undervalued. The aim of this work was to extract cherry seed proteins, to evaluate the presence of bioactive peptides, and to identify them by mass spectrometry. The digestion of cherry seed proteins was optimized, and three different enzymes were employed: Alcalase, Thermolysin, and Flavourzyme. Peptide extracts obtained by the digestion of the cherry seed protein isolate with Alcalase and Thermolysin yielded the highest antioxidant and antihypertensive capacities. Ultrafiltration of hydrolysates allowed obtaining fractions with high antioxidant and antihypertensive capabilities. HPLC-Q-TOF-MS together with bioinformatics tools enabled one to identify peptides in these fractions.

引用

页码：1514 / 1520

页数：7

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