Microsatellites in Cassava (Manihot esculenta Crantz):: discovery, inheritance and variability

被引:103
作者
Chavarriaga-Aguirre, P
Maya, MM
Bonierbale, MW
Kresovich, S
Fregene, MA
Tohme, J
Kochert, G
机构
[1] Univ Georgia, Dept Bot, Athens, GA 30605 USA
[2] CIAT, Cassava Program, Cali, Colombia
[3] Univ Georgia, USDA, ARS, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA
[4] CIAT, Biotechnol Res Unit, Cali, Colombia
关键词
Cassava; microsatellites; fluorescence-based genotyping; heterozygosity; linkage;
D O I
10.1007/s001220050922
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (P-32)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping. Heterozygosity values ranged from 0.00 to 0.88 and the number of alleles detected varied from 1 to 15. The reproducibility of allele sizing was also assessed using fluorescence-based genotyping. The average inter-gel size difference was 1.03 nucleotides. Chi-square tests (chi(2)) were performed to analyse segregation distortion and the linkage between alleles segregating from either or both parents in an F-1 mapping population. Most microsatellite loci segregated in the expected 1:1, 1:2:1 or 1:1:1:1 ratio. Linkage was detected between loci segregating from either parent, and segregation distortion from the male parent was detected for locus GA-131. Approximately 80% of the microsatellites detected one or two alleles per accession, suggesting a low degree of microsatellite locus duplication, an unexpected finding for a putative allopolyploid, highly heterozygous species. The high h values of most microsatellites, their amplification in other Manihot taxa and their suitability for high-throughput, fluorescence-based genotyping, make microsatellites the marker of choice for germplasm characterization and saturation of the cassava map.
引用
收藏
页码:493 / 501
页数:9
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