PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

被引：11

作者：

Benavides, F

Glasscock, E

Coghlan, LG

Stern, MC

Weiss, DA

Conti, CJ

机构：

[1] Univ Texas, MD Anderson Canc Ctr, Div Res, Smithville, TX 78957 USA

[2] Dept Vet Sci, Bastrop, TX 78602 USA

来源：

LABORATORY ANIMALS | 2001年 / 35卷 / 02期

关键词：

genetic monitoring; inbred mice; SENCAR mice; microsatellites;

D O I：

10.1258/0023677011911534

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

引用

页码：157 / 162

页数：6

共 17 条

[1]

Benavides F, 2000, MOL CARCINOGEN, V28, P191, DOI 10.1002/1098-2744(200008)28:4<191::AID-MC1>3.0.CO

[2]

2-K

[3]

Benavides FJ, 1999, CONTEMP TOP LAB ANIM, V38, P54

[4] Development and initial characterization of several new inbred strains of SENCAR mice for studies of multistage skin carcinogenesis [J].

Coghlan, LG ;

Gimenez-Conti, I ;

Kleiner, HE ;

Fischer, SM ;

Rundhaug, JE ;

Conti, CJ ;

Slaga, TJ ;

DiGiovanni, J .

CARCINOGENESIS, 2000, 21 (04) :641-646

[5] COMPARISON OF THE TUMOR-INITIATING ACTIVITY OF 7,12-DIMETHYLBENZ[A]ANTHRACENE AND BENZO[A]PYRENE IN FEMALE SENCAR AND CD-1 MICE [J].

DIGIOVANNI, J ;

SLAGA, TJ ;

BOUTWELL, RK .

CARCINOGENESIS, 1980, 1 (05) :381-389

[6]

FESTING M, 1993, GUIDELINES BREEDING, P65

[7]

Festing M. F. W., 1990, Scandinavian Journal of Laboratory Animal Science, V17, P119

[8]

Festing MFW, 1982, ILAR NEWS, V25, P6

[9] CHARACTERIZATION OF AN INBRED STRAIN OF THE SENCAR MOUSE THAT IS HIGHLY SENSITIVE TO PHORBOL ESTERS [J].

FISCHER, SM ;

OCONNELL, JF ;

CONTI, CJ ;

TACKER, KC ;

FRIES, JW ;

PATRICK, KE ;

ADAMS, LM ;

SLAGA, TJ .

CARCINOGENESIS, 1987, 8 (03) :421-424

[10]

Hennings H, 1997, MOL CARCINOGEN, V20, P143, DOI 10.1002/(SICI)1098-2744(199709)20:1<143::AID-MC16>3.0.CO

← 1 2 →