Metabolic engineering to increase isoflavone biosynthesis in soybean seed

被引：200

作者：

Yu, O ^{[1
]}

Shi, J ^{[1
]}

Hession, AO ^{[1
]}

Maxwell, CA ^{[1
]}

McGonigle, B ^{[1
]}

Odell, JT ^{[1
]}

机构：

[1] DuPont Co Inc, Expt Stn, Crop Genet, Wilmington, DE 19880 USA

来源：

PHYTOCHEMISTRY | 2003年 / 63卷 / 07期

关键词：

Glycine max; fabaceae; isoflavone; transcription factor; flavanone; 3-hydroxylase;

D O I：

10.1016/S0031-9422(03)00345-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isoflavone levels in Glycine max (soybean) were increased via metabolic engineering of the complex phenylpropanoid biosynthetic pathway. Phenylpropanoid pathway genes were activated by expression of the maize Cl and R transcription factors in soybean seed, which decreased genistein and increased the daidzein levels with a small overall increase in total isoflavone levels. Cosuppression of flavanone 3-hydroxylase to block the anthocyanin branch of the pathway, in conjunction with C1/R expression, resulted in higher levels of isoflavones. The combination of transcription factor-driven gene activation and suppression of a competing pathway provided a successful means of enhancing accumulation of isoflavones in soybean seed. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：753 / 763

页数：11

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