Expression of Netrin-1 and its two receptors DCC and UNC5H2 in the developing mouse lung

被引:36
作者
Dalvin, S
Anselmo, MA
Prodhan, P
Komatsuzaki, K
Schnitzer, JJ
Kinane, TB [1 ]
机构
[1] Harvard Univ, Massachusetts Gen Hosp Children, Sch Med, Dept Pediat,Pediat Pulm Unit, Boston, MA 02114 USA
[2] Harvard Univ, Massachusetts Gen Hosp Children, Sch Med,Pediat Surg Serv, Pediat Surg Res Lab, Boston, MA 02114 USA
[3] Harvard Univ, Massachusetts Gen Hosp Children, Sch Med, Dept Surg, Boston, MA 02114 USA
基金
美国国家卫生研究院;
关键词
Netrin; UNC5; DCC; lung; development; branching morphogenesis; quantitative reverse transcription-polymerase chain reaction; mouse;
D O I
10.1016/S1567-133X(03)00047-4
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The ligand Netrin-1 and its receptors DCC and UNC5H2 are critical for the regulation of neuronal migration in nervous system development. Here we demonstrate expression of these molecules in lung development. The mRNA expression profiles of Netrin-1, DCC and UNC5H2 are developmentally regulated during embryonic mouse lung formation. Netrin-1 shows a bimodal expression pattern with elevated mRNA levels early followed by a second peak in late gestation. Peak expression of DCC occurs early in development whereas expression of UNC5H2 peaks late in development. We also demonstrate localization of Netrin-1, DCC and UNC5H2 during the stages of lung development. We present evidence that these proteins are modulated spatially in the mesenchyme and epithelium during lung organogenesis. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:279 / 283
页数:5
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