Nanoscale-controlled spacing provides DNA microarrays with the SNP discrimination efficiency in solution phase

被引：86

作者：

Hong, BJ ^{[1
]}

Oh, SJ ^{[1
]}

Youn, TO ^{[1
]}

Kwon, SH ^{[1
]}

Park, JW ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Div Mol & Life Sci, Dept Chem, Ctr Integrated Mol Syst, Pohang 790784, South Korea

来源：

LANGMUIR | 2005年 / 21卷 / 10期

关键词：

D O I：

10.1021/la046951y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have prepared solid substrates modified with a cone-shaped dendron that generates mesospacing (3.2 nm on average) on the surface. This nanoscale-controlled surface provided an ideal DNA microarray in which each probe DNA strand was given ample space for the incoming target DNA, resulting in selectivity as high as that in solution (100: < 1). In addition, high hybridization yield confirms that DNA probes on the mesospaced surface are sterically unhindered for the hybridization.

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页码：4257 / 4261

页数：5

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