Micromanipulation of the Chlamydia pneumoniae inclusion:: implications for cloning and host-pathogen interactions

被引：3

作者：

Gieffers, J ^{[1
]}

Tamplin, V

Maass, M

Belland, RJ

Caldwell, HD

机构：

[1] NIAID, Intracellular Parasites Lab, NIH, Hamilton, MT 59840 USA

[2] Med Univ Lubeck, Inst Med Microbiol & Hyg, D-23538 Lubeck, Germany

[3] NIAID, Microscopy Branch, NIH, Hamilton, MT 59840 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 226卷 / 01期

关键词：

micromanipulation; microinjection; mycoplasma; cloning; Chlamydia;

D O I：

10.1016/S0378-1097(03)00563-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

The Chlamydia trachomatis inclusion is fragile, rendering it incompatible to micromanipulation. We show that the Chlamydia pneumoniae inclusion differs, being resistant to micromanipulation as shown by direct microinjection of the infected host cytosol or the inclusion itself. We have used micromanipulation to clone C pneumoniae and to free it from mycoplasma contamination. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：45 / 49

页数：5

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