Needle puncture injury affects intervertebral disc mechanics and biology in an organ culture model

被引:154
作者
Korecki, Casey L. [1 ]
Costi, John J. [1 ,2 ,3 ]
Iatridis, James C. [1 ]
机构
[1] Univ Vermont, Coll Engn & Math Sci, Sch Engn, Burlington, VT USA
[2] Flinders Univ S Australia, Repatriat Gen Hosp, Dept Orthopaed, Adelaide, SA 5001, Australia
[3] Flinders Univ S Australia, Adelaide, SA 5001, Australia
关键词
spine; intervertebral disc; organ culture; needle puncture; bovine; discography; mechanics; dynamic compression loading;
D O I
10.1097/BRS.0b013e3181624504
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Study Design. A bovine intervertebral disc organ culture model was used to study the effect of needle puncture injury on short-term disc mechanics and biology. Objective. To test the hypothesis that significant changes in intervertebral disc structure, mechanics, and cellular response would be present within 1 week of needle puncture injury with a large-gauge needle but not with a small-gauge needle. Summary of Background Data. Defects in anulus fibrosus induced by needle puncture injury can compromise mechanical integrity of the disc and lead to degeneration in animal models. The immediate and short-term mechanical and biologic response to anulus injury through needle puncture in a large animal model is not known. Methods. Bovine caudal intervertebral discs were harvested, punctured posterolaterally using 25G and 14G needles, and placed in organ culture for 6 days. Discs underwent a daily dynamic compression loading protocol for 5 days from 0.2 to 1 MPa at 1 Hz for 1 hour. Disc structure and function were assessed with measurements of dynamic modulus, creep, height loss, water content, proteoglycan loss to the culture medium, cell viability, and histology. Results. Needle puncture injury caused a rapid decrease in dynamic modulus and increase in creep during 1-hour loading, although no changes were detected in water content, disc height, or proteoglycan lost to the media. Cell viability was maintained except for localized cell death at the needle insertion site. An increase in cell number and possible remodeling response was seen in the insertion site in the nucleus pulposus. Conclusion. Relatively minor disruption in the disc from needle puncture injury had immediate and progressive mechanical and biologic consequences with important implications for the use of discography, and repair-regeneration techniques. Results also suggest diagnostic techniques sensitive to mechanical changes in the disc may be important for early detection of degenerative changes in response to anulus injury.
引用
收藏
页码:235 / 241
页数:7
相关论文
共 35 条
[1]
Mechanical initiation of intervertebral disc degeneration [J].
Adams, MA ;
Freeman, BJC ;
Morrison, HP ;
Nelson, IW ;
Dolan, P .
SPINE, 2000, 25 (13) :1625-1636
[2]
What is intervertebral disc degeneration, and what causes it? [J].
Adams, Michael A. ;
Roughley, Peter J. .
SPINE, 2006, 31 (18) :2151-2161
[3]
Intradiscal administration of osteogenic protein-1 increases intervertebral disc height and proteoglycan content in the nucleus pulposus in normal adolescent rabbits [J].
An, HS ;
Takegami, K ;
Kamada, H ;
Nguyen, CM ;
Thonar, EJMA ;
Singh, K ;
Andersson, GB ;
Masuda, K .
SPINE, 2005, 30 (01) :25-31
[4]
Cellular therapy for disc degeneration [J].
Anderson, DG ;
Albert, TJ ;
Fraser, JK ;
Risbud, M ;
Wuisman, P ;
Meisel, HJ ;
Tannoury, C ;
Shapiro, I ;
Vaccaro, AR .
SPINE, 2005, 30 (17) :S14-S19
[5]
A gold standard evaluation of the "discogenic pain" diagnosis as determined by provocative discography [J].
Carragee, Eugene J. ;
Lincoln, Todd ;
Parmar, Vik Singh ;
Alamin, Todd .
SPINE, 2006, 31 (18) :2115-2123
[6]
IMPROVED QUANTITATION AND DISCRIMINATION OF SULFATED GLYCOSAMINOGLYCANS BY USE OF DIMETHYLMETHYLENE BLUE [J].
FARNDALE, RW ;
BUTTLE, DJ ;
BARRETT, AJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 883 (02) :173-177
[7]
Mechanical and pathologic consequences of induced concentric anular tears in an ovine model [J].
Fazzalari, NL ;
Costi, JJ ;
Hearn, TC ;
Fraser, RD ;
Vernon-Roberts, B ;
Hutchinson, J ;
Manthey, BA ;
Parkinson, IH ;
Sinclair, C .
SPINE, 2001, 26 (23) :2575-2581
[8]
Three-dimensional culture of human disc cells within agarose or a collagen sponge: assessment of proteoglycan production [J].
Gruber, HE ;
Hoelscher, GL ;
Leslie, K ;
Ingram, JA ;
Hanley, EN .
BIOMATERIALS, 2006, 27 (03) :371-376
[9]
An improved staining method for intervertebral disc tissue [J].
Gruber, HE ;
Ingram, J ;
Hanley, EN .
BIOTECHNIC & HISTOCHEMISTRY, 2002, 77 (02) :81-83
[10]
Intervertebral disc mechanics are restored following cyclic loading and unloaded recovery [J].
Johannessen, W ;
Vresilovic, EJ ;
Wright, AC ;
Elliott, DM .
ANNALS OF BIOMEDICAL ENGINEERING, 2004, 32 (01) :70-76