Surface-enhanced Raman spectroscopy as a tool for probing specific biochemical components in bacteria

被引：200

作者：

Zeiri, L

Bronk, BV

Shabtai, Y

Eichler, J

Efrima, S

机构：

[1] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel

[2] AFRL HEST, Aberdeen Proving Ground, MD 21010 USA

[3] Tufts Univ, Ctr Bioengn, Medford, MA 02155 USA

[4] Tufts Univ, Ilse Katz Ctr Meso & Nanoscale Sci & Technol, Medford, MA 02155 USA

[5] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel

来源：

APPLIED SPECTROSCOPY | 2004年 / 58卷 / 01期

关键词：

surface-enhanced Raman scattering; bacteria; flavins; silver colloids;

D O I：

10.1366/000370204322729441

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.

引用

页码：33 / 40

页数：8

共 92 条

[1] Surface-enhanced Raman spectroscopy of insect nuclear polyhedrosis virus [J].