A Burkholderia pseudomallei Toxin Inhibits Helicase Activity of Translation Factor eIF4A

被引：81

作者：

Cruz-Migoni, Abimael ^{[1
]}

Hautbergue, Guillaume M. ^{[1
]}

Artymiuk, Peter J. ^{[1
]}

Baker, Patrick J. ^{[1
]}

Bokori-Brown, Monika ^{[2
]}

Chang, Chung-Te ^{[1
]}

Dickman, Mark J. ^{[3
]}

Essex-Lopresti, Angela ^{[4
]}

Harding, Sarah V. ^{[4
]}

Mahadi, Nor Muhammad ^{[5
]}

Marshall, Laura E. ^{[4
]}

Mobbs, George W. ^{[1
]}

Mohamed, Rahmah ^{[5
,6
]}

Nathan, Sheila ^{[5
,6
]}

Ngugi, Sarah A. ^{[4
]}

Ong, Catherine ^{[7
]}

Ooi, Wen Fong ^{[8
]}

Partridge, Lynda J. ^{[1
]}

Phillips, Helen L. ^{[3
]}

Raih, M. Firdaus ^{[6
]}

Ruzheinikov, Sergei ^{[1
]}

Sarkar-Tyson, Mitali ^{[4
]}

Sedelnikova, Svetlana E. ^{[1
]}

Smither, Sophie J. ^{[4
]}

Tan, Patrick ^{[8
]}

Titball, Richard W. ^{[2
]}

Wilson, Stuart A. ^{[1
]}

Rice, David W. ^{[1
]}

机构：

[1] Univ Sheffield, Krebs Inst, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England

[2] Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4QD, Devon, England

[3] Univ Sheffield, Dept Chem & Biol Engn, Chem Engn Life Sci Interface Inst, Sheffield S1 3JD, S Yorkshire, England

[4] Def Sci & Technol Lab, Salisbury SP4 OJQ, Wilts, England

[5] Malaysia Genome Inst, Kajang 43000, Selangor DE, Malaysia

[6] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Biosci & Biotechnol, Bangi 43600, Selangor DE, Malaysia

[7] Def Med & Environm Res Inst, DSO Natl Labs, Singapore 117510, Singapore

[8] Genome Inst Singapore, Singapore 138672, Singapore

来源：

SCIENCE | 2011年 / 334卷 / 6057期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

INITIATION; RNA; DEAMIDATION; MELIOIDOSIS; ACTIVATION; GLUTAMINE; COMPLEX; FAMILY; RHO;

D O I：

10.1126/science.1211915

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

引用

页码：821 / 824

页数：4

共 16 条

[1]

Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA [J].