Rat astroglial P2Z (P2X(7)) receptors regulate intracellular calcium and purine release

被引：128

作者：

Ballerini, P

Rathbone, MP

DiIorio, P

Renzetti, A

Giuliani, P

DAlimonte, I

Trubiani, O

Caciagli, F

Ciccarelli, R

机构：

[1] MCMASTER UNIV,HLTH SCI CTR,DEPT BIOMED SCI,HAMILTON,ON L8N 3Z5,CANADA

[2] MCMASTER UNIV,HLTH SCI CTR,DEPT MED,HAMILTON,ON L8N 3Z5,CANADA

[3] UNIV CHIETI,INST PHARMACOL & BIOMED TECHNOL,CHIETI,ITALY

[4] UNIV CHIETI,INST HUMAN MORPHOL,CHIETI,ITALY

来源：

NEUROREPORT | 1996年 / 7卷 / 15-17期

关键词：

primary astroglial cultures; extracellular purines; purinoceptors; P2X(7) (P2Z) subtype; membrane permeabilization; Ca2+](i) levels; H-3]purine release;

D O I：

10.1097/00001756-199611040-00026

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

TREATMENT Of rat astrocyte cultures with 2'- and 3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP), a P2X(7) agonist, but not with adenosine 5-[alpha beta methylene] triphosphate (alpha,beta meATP), a P2X agonist, increased influx of extracellular Ca2+ and [Ca2+](i). Lucifer yellow, a small molecule which permeates P2X(7) receptor-induced pores, entered BzATP-treated but not control astrocytes. BzATP also stimulated efflux of [H-3]purine from cultured astrocytes. The P2X(7) receptor antagonist oxidized ATP abolished the effects of BzATP on [Ca2+](i) lucifer yellow permeation and [H-3]purine release, indicating that these effects were due to P2X(7) receptor activation. In neurological diseases or injuries extracellular ATP may activate P2X(7) receptors further enhancing [H-3]purine release, with important pathophysiological consequences.

引用

页码：2533 / 2537

页数：5

共 25 条

[1]

BALLERINI P, 1993, RES COMMUN CHEM PATH, V82, P167

[2]

BALLERINI P, 1995, RES COMMUN MOL PATH, V87, P49

[3]

BALLERINI P, 1995, NEUROCHEM RES, V20, P627

[4] CULTURES OF GLIAL-CELLS RELEASE PURINES UNDER FIELD ELECTRICAL-STIMULATION - THE POSSIBLE IONIC MECHANISMS [J].

CACIAGLI, F ;

CICCARELLI, R ;

DILORIO, P ;

BALLERINI, P ;

TACCONELLI, L .

PHARMACOLOGICAL RESEARCH COMMUNICATIONS, 1988, 20 (11) :935-947

[5] EFFECTS OF EXOGENOUS ATP AND RELATED ANALOGS ON THE PROLIFERATION RATE OF DISSOCIATED PRIMARY CULTURES OF RAT ASTROCYTES [J].

CICCARELLI, R ;

DIIORIO, P ;

BALLERINI, P ;

AMBROSINI, G ;

GIULIANI, P ;

TIBONI, GM ;

CACIAGLI, F .

JOURNAL OF NEUROSCIENCE RESEARCH, 1994, 39 (05) :556-566

[6]

DAZIEL HH, 1994, PHARMACOL REV, V46, P449

[7] FC-RECEPTOR MEDIATED PHAGOCYTOSIS OCCURS IN MACROPHAGES AT EXCEEDINGLY LOW CYTOSOLIC CA-2+ LEVELS [J].

DIVIRGILIO, F ;

MEYER, BC ;

GREENBERG, S ;

SILVERSTEIN, SC .

JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :657-666

[8]

ELMOATASSIM C, 1993, J BIOL CHEM, V268, P15571

[9]

FERRARI D, 1996, J IMMUNOL, V156, P1532

[10]

FIERZ W, 1986, ASTROCYTES CELL BIOL, V3

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