A sensitive detection of phospho-Smad1/5/8 and Smad2 in Western blot analyses

被引：22

作者：

Funaba, Masayuki ^{[1
]}

Murakami, Masaru ^{[2
]}

机构：

[1] Azabu Univ Sch Vet Med, Lab Nutr, Sagamihara, Kanagawa 2298501, Japan

[2] Azabu Univ Sch Vet Med, Mol Biol Lab, Sagamihara, Kanagawa 2298501, Japan

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2008年 / 70卷 / 06期

基金：

日本学术振兴会;

关键词：

Smad; phosphorylation; Western blot;

D O I：

10.1016/j.jbbm.2007.10.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The transforming growth factor-beta (TGF-beta) family is involved in a variety of physiological processes, and transmits signals through phosphorylation of Smad by the receptor complexes. In the present study, effects of blocking solution in Western blot analyses on detection of phosphorylated Smad1/5/8 and Smad2 were examined. When EzBlock was used as a blocking reagent, phosphorylated Smad 1 /8 and Smad2 were most efficiently detected. The anti-phospho-Smad2 antibody specifically recognized the phosphorylated form of Smad2, whereas the antiphospho-Smad1/5/8 antibody also reacted to the unphosphorylated form. These antibodies did not react with the other Smads. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：816 / 819

页数：4

共 4 条

[1]

Specificity and versatility in TGF-β signaling through Smads [J].