Identification of hazelnut (Corylus avellana) cultivars by RAPD analysis

被引：40

作者：

Galderisi, U

Cipollaro, M

Di Bernardo, G

De Masi, L

Galano, G

Cascino, A

机构：

[1] Univ Naples, CRISCEB, Fac Med & Chirurg, I-80138 Naples, Italy

[2] Univ Naples, Ist Farmacol & Tossicol, Fac Med & Chirurg, I-80138 Naples, Italy

来源：

PLANT CELL REPORTS | 1999年 / 18卷 / 7-8期

关键词：

PCR; RAPD analysis; Corylus avellana; cultivars;

D O I：

10.1007/s002990050637

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The random amplified polymorphic DNA (RAPD) technique offers a useful tool to detect DNA polymorphisms. It can also be used to distinguish different clones and cultivars. We have developed a comprehensive RAPD-based procedure for the routine molecular typing of various plants. Here we report the application of this technique for the correct identification of six hazelnut cultivars (Corylus avellana) widespread in the Campania region (south Italy). The analysed hazelnut cultivars were successfully distinguished by their RAPD fingerprints using the DNA primers U2, U3, U4, UI I and U14. However, in each cultivar we observed very low genetic heterogeneity among the clonal variants. Since this technique is among the simplest and easiest: methods used to Fingerprint DNA, it could be easily transferred to less sophisticated laboratory infrastructures (e.g. outstations of crop regulatory agencies).

引用

页码：652 / 655

页数：4

共 16 条

[1]

[Anonymous], FINGERPRINTING METHO

[2] INTRASPECIFIC FLAVONOID VARIATION [J].