Umbilical cord mesenchymal stem cells suppress B-cell proliferation and differentiation

被引：107

作者：

Che, Nan ^{[2
]}

Li, Xia ^{[2
]}

Zhou, Shiliang ^{[3
]}

Liu, Rui ^{[2
]}

Shi, Dongyan ^{[3
]}

Lu, Liwei ^{[1
,4
]}

Sun, Lingyun ^{[2
]}

机构：

[1] Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China

[2] Nanjing Med Univ, Drum Tower Clin Med Coll, Dept Immunol & Rheumatol, Nanjing, Jiangsu, Peoples R China

[3] Nanjing Univ, Sch Med, Affiliated Drum Tower Hosp, Dept Immunol & Rheumatol, Nanjing, Jiangsu, Peoples R China

[4] Univ Hong Kong, Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China

来源：

CELLULAR IMMUNOLOGY | 2012年 / 274卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Umbilical cord mesenchymal stem cells; B cells; B-cell proliferation and differentiation; HUMAN BONE-MARROW; GERMINAL-CENTER FORMATION; REPRESSION; TRANSPLANTATION; ACTIVATION; PAX5; TRANSCRIPTION; EXPRESSION; BLIMP-1; MODEL;

D O I：

10.1016/j.cellimm.2012.02.004

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Mesenchymal stem cells (MSCs) may be obtained from umbilical cord as an abundant and noninvasive source. However, the immunomodulatory properties of umbilical cord-MSCs (UC-MSCs) were poorly studied. In this study, we aimed to investigate the effects of UC-MSCs on B-cell proliferation and differentiation. UC-MSCs were found to suppress the proliferation of B cells isolated from murine spleen. Moreover, UC-MSCs markedly suppressed B-cell differentiation as shown by the decreased number of CD138 + cells and reduced levels of IgM and IgG production in coculture. As revealed by transwell experiments, soluble factors produced by UC-MSCs might be involved in mediating B-cell suppression. The Blimp-1 mRNA expression was suppressed whereas the PAX-5 mRNA expression was induced in coculture. Finally, UC-MSCs modified the phosphorylation pattern of Akt and p38 pathways, which were involved in B-cell proliferation and differentiation. These results may further support the potential therapeutic use of UC-MSCs in treating autoimmune disorders. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：46 / 53

页数：8

共 49 条

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BANK negatively regulates Akt activation and subsequent B cell responses [J].