Cloning, purification, crystallization and preliminary crystallographic analysis of a hypothetical acetyltransferase from Pyrococcus furiosus

被引:5
作者
Biarrotte-Sorin, S
Mayer, C [1 ]
机构
[1] CHU Pitie Salpetriere, Lab Rech Mol Antibiot, Paris, France
[2] Lab Mineral Cristallog Paris, Paris, France
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词
D O I
10.1107/S174430910500223X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The GCN5-related N-acetyltransferase (GNAT) superfamily has a primordial role in cellular processes such as transcripition initiation and regulation by histone acetylation, aminoglycoside resistance and melatonin metabolism. To date, no acetyltransferase from the archaeal domain of life has been studied. This paper describes the cloning, expression, purification and crystallization of a Pyrococcus Furiosus hypothetical acetyltransferase PfGNAT (MW = 22 007 Da). The crystals belong to space group P622, with one molecule in the asymmetric unit and unit-cell parameters a = b = 82.6, c = 105.92 angstrom, alpha = beta = 90, gamma = 120 degrees. Crystals diffract X-rays to 30 angstrom resolution on a synchroton-radiation source. Determination of this structure will provide new insights into substrate-specificity of this acetyltransferase and the thermal stability of the N-acetyltransferase domain.
引用
收藏
页码:269 / 270
页数:2
相关论文
共 12 条
  • [1] BARROTTESORIN S, 2004, STRUCTURE, V12, P1
  • [2] GCN5-related N-acetyltransferases: A structural overview
    Dyda, F
    Klein, DC
    Hickman, AB
    [J]. ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2000, 29 : 81 - 103
  • [3] Histone acetylation in chromatin structure and transcription
    Grunstein, M
    [J]. NATURE, 1997, 389 (6649) : 349 - 352
  • [4] AUTOMATIC-INDEXING OF ROTATION DIFFRACTION PATTERNS
    KABSCH, W
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1988, 21 : 67 - 71
  • [5] AMORE - AN AUTOMATED PACKAGE FOR MOLECULAR REPLACEMENT
    NAVAZA, J
    [J]. ACTA CRYSTALLOGRAPHICA SECTION A, 1994, 50 : 157 - 163
  • [6] GCN5-related histone N-acetyltransferases belong to a diverse superfamily that includes the yeast SPT10 protein
    Neuwald, AF
    Landsman, D
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (05) : 154 - 155
  • [7] Robb FT, 2001, METHOD ENZYMOL, V330, P134
  • [8] FIP:: a highly automated beamline for multiwavelength anomalous diffraction experiments
    Roth, M
    Carpentier, P
    Kaïkati, O
    Joly, J
    Charrault, P
    Pirocchi, M
    Kahn, R
    Fanchon, E
    Jacquamet, L
    Borel, F
    Bertoni, A
    Israel-Gouy, P
    Ferrer, JL
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2002, 58 : 805 - 814
  • [9] Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT, family of N-acetyltransferases
    Vetting, MW
    Roderick, SL
    Yu, M
    Blanchard, JS
    [J]. PROTEIN SCIENCE, 2003, 12 (09) : 1954 - 1959
  • [10] Crystal structure of the anti-fungal target N-myristoyl transferase
    Weston, SA
    Camble, R
    Colls, J
    Rosenbrock, G
    Taylor, I
    Egerton, M
    Tucker, AD
    Tunnicliffe, A
    Mistry, A
    Mancia, F
    de la Fortelle, E
    Irwin, J
    Bricogne, G
    Pauptit, RA
    [J]. NATURE STRUCTURAL BIOLOGY, 1998, 5 (03) : 213 - 221