Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1

被引:442
作者
Geisler, M
Blakeslee, JJ
Bouchard, R
Lee, OR
Vincenzetti, V
Bandyopadhyay, A
Titapiwatanakun, B
Peer, WA
Bailly, A
Richards, EL
Ejenda, KFK
Smith, AP
Baroux, C
Grossniklaus, U
Müller, A
Hrycyna, CA
Dudler, R
Murphy, AS [1 ]
Martinoia, E
机构
[1] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA
[2] Univ Zurich, Inst Plant Biol, Basel Zurich Plant Sci Ctr, CH-8007 Zurich, Switzerland
[3] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[4] Ruhr Univ Bochum, Lehrstuhl Pflanzenphysiol, D-44801 Bochum, Germany
关键词
auxin; P-glycoprotein; multi-drug resistance; ABC transporter;
D O I
10.1111/j.1365-313X.2005.02519.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Directional transport of the phytohormone auxin is required for the establishment and maintenance of plant polarity, but the underlying molecular mechanisms have not been fully elucidated. Plant homologs of human multiple drug resistance/P-glycoproteins (MDR/PGPs) have been implicated in auxin transport, as defects in MDR1 (AtPGP19) and AtPGP1 result in reductions of growth and auxin transport in Arabidopsis (atpgp1, atpgp19), maize (brachytic2) and sorghum (dwarf3). Here we examine the localization, activity, substrate specificity and inhibitor sensitivity of AtPGP1. AtPGP1 exhibits non-polar plasma membrane localization at the shoot and root apices, as well as polar localization above the root apex. Protoplasts from Arabidopsis pgp1 leaf mesophyll cells exhibit reduced efflux of natural and synthetic auxins with reduced sensitivity to auxin efflux inhibitors. Expression of AtPGP1 in yeast and in the standard mammalian expression system used to analyze human MDR-type proteins results in enhanced efflux of indole-3-acetic acid (IAA) and the synthetic auxin 1-naphthalene acetic acid (1-NAA), but not the inactive auxin 2-NAA. AtPGP1-mediated efflux is sensitive to auxin efflux and ABC transporter inhibitors. As is seen in planta, AtPGP1 also appears to mediate some efflux of IAA oxidative breakdown products associated with apical sites of high auxin accumulation. However, unlike what is seen in planta, some additional transport of the benzoic acid is observed in yeast and mammalian cells expressing AtPGP1, suggesting that other factors present in plant tissues confer enhanced auxin specificity to PGP-mediated transport.
引用
收藏
页码:179 / 194
页数:16
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