Brain maturation of high-affinity adenosine A(2) receptors and their coupling to G-proteins

被引:10
作者
Doriat, JF [1 ]
Humbert, AC [1 ]
Daval, JL [1 ]
机构
[1] UNIV NANCY 1,INSERM,U272,F-54013 NANCY,FRANCE
来源
DEVELOPMENTAL BRAIN RESEARCH | 1996年 / 93卷 / 1-2期
关键词
rat striatum; postnatal development; adenosine A(2) receptor; autoradiography; CGS; 21680; binding;
D O I
10.1016/0165-3806(96)00009-0
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The neuromodulator adenosine is acting through specific receptors, A(1) and A(2), coupled to their effector systems via G-proteins. The regulatory effects of adenosine on locomotor activity have been attributed to an interaction with A(2) striatal receptors. The postnatal development of adenosine A(2a) receptors was analysed in rat striatal membranes and by quantitative autoradiography in brain sections using [H-3]CGS 21680 as specific probe. At the concentration of radioligand used (5 nM), A(2a) sites were concentrated in the striatum at all ages, with minor developmental alterations in the expression pattern within the striatal regions. In membrane preparations, Scatchard analysis showed that the density of CGS 21680 binding sites was low at birth, around 3% of the adult value, and then increased, mostly between birth and 5 days and then from 15 days to adulthood. Concomitantly, the receptor affinity decreased sharply during brain development, K-d values varying from 2 to 15.5 nM. The addition of a GTP analogue, guanylyl-5'-imidodiphosphate (Gpp(NH)p, 10 mu M), to the assay medium reduced significantly the receptor affinity throughout the postnatal development, reflecting a coupling to G-proteins at all ages, but it also suggested a weaker association at birth. These data show that the developmental properties of A(2a) receptors contrast with those of A(1) receptors, and emphasize the role played by adenosine through its A(2) receptors in the maturation of striatum-related cerebral pathways.
引用
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页码:1 / 9
页数:9
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