Antineoplastic Effects of Gamma Linolenic Acid on Hepatocellular Carcinoma Cell Lines

被引：30

作者：

Itoh, Shinji ^{[1
]}

Taketomi, Akinobu ^{[1
]}

Harimoto, Norifumi ^{[1
]}

Tsujita, Eiji ^{[1
]}

Rikimaru, Tatsuya ^{[1
]}

Shirabe, Ken ^{[1
]}

Shimada, Mitsuo ^{[2
]}

Maehara, Yoshihiko ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Surg & Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] Univ Tokushima, Grad Sch Med, Dept Digest & Pediat Surg, Tokushima 7708503, Japan

来源：

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION | 2010年 / 47卷 / 01期

关键词：

gamma linolenic acid; hepatocellular carcinoma; oxidant stress; heme oxygenase-1; OXIDATIVE STRESS; HEME OXYGENASE; VITAMIN-E; EXPRESSION; APOPTOSIS; THIOREDOXIN; REDUCTASE; BILIRUBIN; THERAPY; KINASE;

D O I：

10.3164/jcbn.10-24

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The aim of this study was to investigate the effect and the mechanism of gamma linolenic acid (GLA) treatment on human hepatocellular (HCC) cell lines. The human HCC cell line HuH7 was exposed to GLA. Cell proliferation and reactive oxygen species (ROS) generation including lipid peroxidation and apoptosis were compared. We then used a cDNA microarray analysis to investigate the molecular changes induced by GLA. GLA treatment significantly reduced cell proliferation, generated ROS, and induced apoptosis. After 24 h exposure of Huh7 cells to GLA, we identified several genes encoding the antioxidant proteins to be upregulated: heme oxygenase-1 (HO-1), aldo-keto reductase 1 family C1 (AKR1C1), C4 (AKR1C4), and thioredoxin (Trx). The HO-1 protein levels were overexpressed in Huh7 cells after GLA exposure using a Western blot analysis. Furthermore, chromium mesoporphyrin (CrMP), an inhibitor of HO activity, significantly potentiated GLA cytotoxicity. GLA treatment has induced cell growth inhibition, ROS generation including lipid peroxidation, and HO-1 production for antioxidant protection against oxidative stress caused by GLA in Huh7 cells. GLA treatment should be considered as a therapeutic modality in patients with advanced HCC.

引用

页码：81 / 90

页数：10

共 33 条

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